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SART3 antibody - middle region

Rabbit Polyclonal Antibody

     
  • WB - SART3 antibody - middle region AI10125
    SART3 antibody - middle region (AI10125) in Human Small Intestine cells using Western Blot
    WB Suggested Anti-SART3 Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:62500
    Positive Control: Human Small Intestine
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q15020
Other Accession Q15020, NP_055521, NM_014706
Reactivity Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Dog, Guinea Pig, Horse, Bovine, Yeast
Predicted Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Chicken, Dog, Guinea Pig, Horse, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 110 kDa
Additional Information
Alias Symbol KIAA0156, MGC138188, RP11-13G14, TIP110, p110(nrb), P100, p110, DSAP1
Other Names Squamous cell carcinoma antigen recognized by T-cells 3, SART-3 {ECO:0000312|EMBL:BAA783841}, Tat-interacting protein of 110 kDa, Tip110, p110 nuclear RNA-binding protein, SART3 (HGNC:16860)
Target/Specificity SART3 is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. SART3 is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This protein is thought to be involved in the regulation of mRNA splicing. The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-SART3 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
PrecautionsSART3 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SART3 (HGNC:16860)
Function U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation (PubMed:12032085). Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites (PubMed:14749385). May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly (PubMed:20595234). May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair (PubMed:24526689). May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC (By similarity).
Cellular Location Nucleus, nucleoplasm. Nucleus, Cajal body. Nucleus speckle. Cytoplasm
Tissue Location Ubiquitously expressed. EMBL; AB020880; BAA78384.1; -; mRNA EMBL; AF387506; AAK69347.1; -; mRNA EMBL; D63879; BAA09929.1; -; mRNA EMBL; AK290209; BAF82898.1; -; mRNA EMBL; AC008119; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AC010206; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; KF455716; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; KF511170; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC032601; AAH32601.1; -; mRNA EMBL; BC041638; AAH41638.1; -; mRNA EMBL; BC093784; AAH93784.1; -; mRNA EMBL; BC103706; AAI03707.1; -; mRNA EMBL; BC111983; AAI11984.1; -; mRNA EMBL; BC143253; AAI43254.1; -; mRNA CCDS; CCDS9117.1; -. [Q15020-1] RefSeq; NP_055521.1; NM_014706.3. [Q15020-1] UniGene; Hs.584842; - PDB; 2DO4; NMR; -; A=791-877 PDB; 5CTQ; X-ray; 2.60 A; A/B/C/D=94-611 PDB; 5CTR; X-ray; 3.01 A; A/B=278-611 PDB; 5CTT; X-ray; 1.70 A; B=601-649 PDB; 5JJW; X-ray; 3.01 A; A=280-578 PDB; 5JJX; X-ray; 2.00 A; A=81-393 PDB; 5JPZ; X-ray; 3.04 A; A/B=96-574 PDBsum; 2DO4; - PDBsum; 5CTQ; - PDBsum; 5CTR; - PDBsum; 5CTT; - PDBsum; 5JJW; - PDBsum; 5JJX; - PDBsum; 5JPZ; - ProteinModelPortal; Q15020; - SMR; Q15020; - BioGrid; 115082; 194 IntAct; Q15020; 53 MINT; Q15020; - STRING; 9606.ENSP00000228284; - iPTMnet; Q15020; - PhosphoSitePlus; Q15020; - BioMuta; SART3; - DMDM; 74762140; - EPD; Q15020; - MaxQB; Q15020; - PaxDb; Q15020; - PeptideAtlas; Q15020; - PRIDE; Q15020; - ProteomicsDB; 60370; - ProteomicsDB; 60371; -. [Q15020-2] ProteomicsDB; 60372; -. [Q15020-3] Ensembl; ENST00000228284; ENSP00000228284; ENSG00000075856. [Q15020-1] Ensembl; ENST00000431469; ENSP00000414453; ENSG00000075856. [Q15020-4] Ensembl; ENST00000546611; ENSP00000448554; ENSG00000075856. [Q15020-3] Ensembl; ENST00000546728; ENSP00000449743; ENSG00000075856. [Q15020-2] GeneID; 9733; - KEGG; hsa:9733; - UCSC; uc001tmz.2; human. [Q15020-1] CTD; 9733; - DisGeNET; 9733; - EuPathDB; HostDB:ENSG00000075856.11; - GeneCards; SART3; - HGNC; HGNC:16860; SART3 HPA; HPA044322; - MIM; 611684; gene neXtProt; NX_Q15020; - OpenTargets; ENSG00000075856; - PharmGKB; PA34948; - eggNOG; KOG0128; Eukaryota eggNOG; ENOG410XP88; LUCA GeneTree; ENSGT00900000141107; - HOGENOM; HOG000063708; - HOVERGEN; HBG053888; - InParanoid; Q15020; - KO; K22611; - OMA; SQAVMKM; - OrthoDB; EOG091G041L; - PhylomeDB; Q15020; - TreeFam; TF317554; - ChiTaRS; SART3; human EvolutionaryTrace; Q15020; - GeneWiki; SART3; - GenomeRNAi; 9733; - PRO; PR:Q15020; - Proteomes; UP000005640; Chromosome 12 Bgee; ENSG00000075856; - CleanEx; HS_SART3; - ExpressionAtlas; Q15020; baseline and differential Genevisible; Q15020; HS GO; GO:0015030; C:Cajal body; IDA:UniProtKB GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell GO; GO:0005654; C:nucleoplasm; IDA:UniProtKB GO; GO:0005634; C:nucleus; IDA:UniProtKB GO; GO:0042393; F:histone binding; IDA:UniProtKB GO; GO:0003723; F:RNA binding; HDA:UniProtKB GO; GO:0030621; F:U4 snRNA binding; IDA:UniProtKB GO; GO:0017070; F:U6 snRNA binding; IDA:UniProtKB GO; GO:0030624; F:U6atac snRNA binding; IDA:UniProtKB GO; GO:1990381; F:ubiquitin-specific protease binding; IPI:UniProtKB GO; GO:0000902; P:cell morphogenesis; IEA:Ensembl GO; GO:0071425; P:hematopoietic stem cell proliferation; IEA:Ensembl GO; GO:0048872; P:homeostasis of number of cells; IEA:Ensembl GO; GO:0000398; P:mRNA splicing, via spliceosome; IDA:UniProtKB GO; GO:0006334; P:nucleosome assembly; IDA:UniProtKB GO; GO:1903586; P:positive regulation of histone deubiquitination; IDA:UniProtKB GO; GO:0010468; P:regulation of gene expression; IDA:MGI GO; GO:0000387; P:spliceosomal snRNP assembly; IDA:UniProtKB GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IDA:UniProtKB CDD; cd12391; RRM1_SART3; 1 CDD; cd12392; RRM2_SART3; 1 Gene3D; 1.25.40.10; -; 2 Gene3D; 3.30.70.330; -; 2 InterPro; IPR003107; HAT InterPro; IPR008669; LSM_interact InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf InterPro; IPR035979; RBD_domain_sf InterPro; IPR000504; RRM_dom InterPro; IPR034217; SART3_RRM1 InterPro; IPR034218; SART3_RRM2 InterPro; IPR011990; TPR-like_helical_dom_sf Pfam; PF05391; Lsm_interact; 1 Pfam; PF00076; RRM_1; 2 SMART; SM00386; HAT; 7 SMART; SM00360; RRM; 2 SUPFAM; SSF48452; SSF48452; 1 SUPFAM; SSF54928; SSF54928; 1 PROSITE; PS50102; RRM; 2 1: Evidence at protein level; 3D-structure; Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Methylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; RNA-binding INIT_MET 1 1 Removed. {ECO:0000244|PubMed:19369195, ECO:0000244|PubMed:19413330, ECO:0000244|PubMed:20068231, ECO:0000244|PubMed:21406692, ECO:0000244|PubMed:22223895, ECO:0000244|PubMed:22814378, ECO:0000269|Ref.13} CHAIN 2 963 Squamous cell carcinoma antigen recognized by T-cells 3 /FTId=PRO_0000223313 REPEAT 126 158 HAT 1 REPEAT 164 195 HAT 2 REPEAT 201 237 HAT 3 REPEAT 242 275 HAT 4 REPEAT 324 356 HAT 5 REPEAT 359 391 HAT 6 REPEAT 394 430 HAT 7 REPEAT 487 520 HAT 8 DOMAIN 704 782 RRM 1. {ECO:0000255|PROSITE- ProRule:PRU00176} DOMAIN 801 878 RRM 2. {ECO:0000255|PROSITE- ProRule:PRU00176} REGION 2 351 Mediates interaction with PRPF3 REGION 487 520 Required for interaction with USP4 REGION 537 953 Necessary and sufficient for U6 snRNA binding. REGION 600 670 Required for nuclear localization COILED 21 46 COILED 82 110 COILED 559 619 MOTIF 601 617 Nuclear localization signal COMPBIAS 89 92 Poly-Glu COMPBIAS 612 616 Poly-Lys MOD_RES 2 2 N-acetylalanine {ECO:0000244|PubMed:19369195, ECO:0000244|PubMed:19413330, ECO:0000244|PubMed:20068231, ECO:0000244|PubMed:21406692, ECO:0000244|PubMed:22223895, ECO:0000244|PubMed:22814378, ECO:0000269|Ref.13} MOD_RES 10 10 Phosphoserine MOD_RES 16 16 Phosphoserine MOD_RES 215 215 Phosphoserine MOD_RES 650 650 Phosphoserine MOD_RES 657 657 Phosphothreonine MOD_RES 769 769 Phosphoserine MOD_RES 795 795 Phosphoserine MOD_RES 852 852 Phosphoserine MOD_RES 906 906 Omega-N-methylarginine VAR_SEQ 105 129 LSINVYDYNCHVDLIRLLRLEGELT -> VGPGVGSGHLPV FQVLGSPCPGPPP (in isoform 3) /FTId=VSP_017248 VAR_SEQ 130 963 Missing (in isoform 3) /FTId=VSP_017249 VAR_SEQ 351 364 SQYLDRQLKVKDLV -> RSTTESKGFGFICT (in isoform 2) /FTId=VSP_017250 VAR_SEQ 365 963 Missing (in isoform 2) /FTId=VSP_017251 VAR_SEQ 401 436 Missing (in isoform 4) /FTId=VSP_057284 VARIANT 23 23 D -> E (in dbSNP:rs2072579) /FTId=VAR_038802 VARIANT 591 591 V -> M (found in a patient with disseminated superficial actinic porokeratosis; unknown pathological significance; dbSNP:rs118203954) /FTId=VAR_038683 VARIANT 621 621 E -> D (in dbSNP:rs2287546) /FTId=VAR_038684 HELIX 91 107 {ECO:0000244|PDB:5JJX} HELIX 112 124 {ECO:0000244|PDB:5JJX} HELIX 128 141 {ECO:0000244|PDB:5JJX} HELIX 146 159 {ECO:0000244|PDB:5JJX} HELIX 163 176 {ECO:0000244|PDB:5JJX} HELIX 183 195 {ECO:0000244|PDB:5JJX} TURN 196 198 {ECO:0000244|PDB:5JJX} HELIX 202 217 {ECO:0000244|PDB:5JJX} TURN 221 223 {ECO:0000244|PDB:5JJX} HELIX 225 236 {ECO:0000244|PDB:5JJX} STRAND 240 242 {ECO:0000244|PDB:5CTQ} HELIX 245 255 {ECO:0000244|PDB:5JJX} HELIX 262 272 {ECO:0000244|PDB:5JJX} HELIX 279 304 {ECO:0000244|PDB:5JJX} HELIX 310 323 {ECO:0000244|PDB:5JJX} HELIX 326 339 {ECO:0000244|PDB:5JJX} HELIX 344 354 {ECO:0000244|PDB:5JJX} HELIX 361 374 {ECO:0000244|PDB:5JJX} HELIX 379 392 {ECO:0000244|PDB:5JJX} HELIX 396 409 {ECO:0000244|PDB:5CTQ} HELIX 414 429 {ECO:0000244|PDB:5CTQ} STRAND 436 438 {ECO:0000244|PDB:5JJW} HELIX 440 457 {ECO:0000244|PDB:5CTQ} HELIX 459 464 {ECO:0000244|PDB:5CTQ} HELIX 473 484 {ECO:0000244|PDB:5CTQ} HELIX 489 501 {ECO:0000244|PDB:5CTQ} TURN 502 506 {ECO:0000244|PDB:5CTQ} HELIX 508 521 {ECO:0000244|PDB:5CTQ} HELIX 524 537 {ECO:0000244|PDB:5CTQ} HELIX 542 556 {ECO:0000244|PDB:5CTQ} HELIX 559 603 {ECO:0000244|PDB:5CTQ} STRAND 803 807 {ECO:0000244|PDB:2DO4} HELIX 814 821 {ECO:0000244|PDB:2DO4} TURN 822 824 {ECO:0000244|PDB:2DO4} STRAND 827 834 {ECO:0000244|PDB:2DO4} STRAND 840 850 {ECO:0000244|PDB:2DO4} HELIX 851 861 {ECO:0000244|PDB:2DO4} STRAND 864 868 {ECO:0000244|PDB:2DO4} STRAND 870 875 {ECO:0000244|PDB:2DO4} SEQUENCE 963 AA; 109935 MW; 06B26CEB8B19102A CRC64; MATAAETSAS EPEAESKAGP KADGEEDEVK AARTRRKVLS RAVAAATYKT MGPAWDQQEE GVSESDGDEY AMASSAESSP GEYEWEYDEE EEKNQLEIER LEEQLSINVY DYNCHVDLIR LLRLEGELTK VRMARQKMSE IFPLTEELWL EWLHDEISMA QDGLDREHVY DLFEKAVKDY ICPNIWLEYG QYSVGGIGQK GGLEKVRSVF ERALSSVGLH MTKGLALWEA YREFESAIVE AARLEKVHSL FRRQLAIPLY DMEATFAEYE EWSEDPIPES VIQNYNKALQ QLEKYKPYEE ALLQAEAPRL AEYQAYIDFE MKIGDPARIQ LIFERALVEN CLVPDLWIRY SQYLDRQLKV KDLVLSVHNR AIRNCPWTVA LWSRYLLAME RHGVDHQVIS VTFEKALNAG FIQATDYVEI WQAYLDYLRR RVDFKQDSSK ELEELRAAFT RALEYLKQEV EERFNESGDP SCVIMQNWAR IEARLCNNMQ KARELWDSIM TRGNAKYANM WLEYYNLERA HGDTQHCRKA LHRAVQCTSD YPEHVCEVLL TMERTEGSLE DWDIAVQKTE TRLARVNEQR MKAAEKEAAL VQQEEEKAEQ RKRARAEKKA LKKKKKIRGP EKRGADEDDE KEWGDDEEEQ PSKRRRVENS IPAAGETQNV EVAAGPAGKC AAVDVEPPSK QKEKAASLKR DMPKVLHDSS KDSITVFVSN LPYSMQEPDT KLRPLFEACG EVVQIRPIFS NRGDFRGYCY VEFKEEKSAL QALEMDRKSV EGRPMFVSPC VDKSKNPDFK VFRYSTSLEK HKLFISGLPF SCTKEELEEI CKAHGTVKDL RLVTNRAGKP KGLAYVEYEN ESQASQAVMK MDGMTIKENI IKVAISNPPQ RKVPEKPETR KAPGGPMLLP QTYGARGKGR TQLSLLPRAL QRPSAAAPQA ENGPAAAPAV AAPAATEAPK MSNADFAKLF LRK
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Background

This is a rabbit polyclonal antibody against SART3. It was validated on Western Blot using a cell lysate as a positive control. Abgent strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (sales@abgent.com).

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$ 295.00
Cat# AI10125
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