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PARP12 antibody - middle region

Rabbit Polyclonal Antibody

     
  • WB - PARP12 antibody - middle region AI10592
    WB Suggested Anti-PARP12 Antibody Titration: .2-1 μg/ml
    ELISA Titer: 1:625
    Positive Control: ACHN cell lysate

    PARP12 is supported by BioGPS gene expression data to be expressed in ACHN

  • IHC - PARP12 antibody - middle region AI10592
    Rabbit Anti-PARP12 Antibody

    Catalog Number:
    AI10592

    Paraffin Embedded Tissue:
    Human cardiac cell

    Cellular Data:
    Epithelial cells of renal tubule

    Antibody Concentration:
    4.-8. μg/ml

    Magnification:
    4X
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q9H0J9
Other Accession NM_022750, NP_073587
Reactivity Human, Mouse, Rat, Pig, Horse, Bovine, Dog
Predicted Human, Mouse, Pig, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 79kDa
Additional Information
Alias Symbol FLJ22693, MST109, MSTP109, PARP-12, ZC3H1, ZC3HDC1
Other Names Poly [ADP-ribose] polymerase 12, PARP-12, 2.4.2.30, ADP-ribosyltransferase diphtheria toxin-like 12, ARTD12, Zinc finger CCCH domain-containing protein 1, PARP12, ZC3HDC1
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-PARP12 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsPARP12 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PARP12
Synonyms ZC3HDC1
Cellular Location Nucleus. EMBL; AL136766; CAB66700.1; -; mRNA EMBL; AL137255; CAB70657.1; -; mRNA EMBL; AC004849; AAS00360.1; -; Genomic_DNA EMBL; AC025816; AAF66161.1; -; Genomic_DNA EMBL; AC004961; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC081541; AAH81541.1; -; mRNA EMBL; AK026346; BAB15457.1; -; mRNA CCDS; CCDS5857.1; - PIR; T46327; T46327 RefSeq; NP_073587.1; NM_022750.3 UniGene; Hs.12646; - UniGene; Hs.308710; - PDB; 2PQF; X-ray; 2.20 A; A/B/C/D/E/F=489-684 PDBsum; 2PQF; - ProteinModelPortal; Q9H0J9; - SMR; Q9H0J9; - BioGrid; 122274; 19 IntAct; Q9H0J9; 5 STRING; 9606.ENSP00000263549; - BindingDB; Q9H0J9; - ChEMBL; CHEMBL2429709; - iPTMnet; Q9H0J9; - PhosphoSitePlus; Q9H0J9; - BioMuta; PARP12; - DMDM; 47117630; - EPD; Q9H0J9; - MaxQB; Q9H0J9; - PaxDb; Q9H0J9; - PeptideAtlas; Q9H0J9; - PRIDE; Q9H0J9; - ProteomicsDB; 80289; - Ensembl; ENST00000263549; ENSP00000263549; ENSG00000059378 GeneID; 64761; - KEGG; hsa:64761; - UCSC; uc003vvl.2; human CTD; 64761; - DisGeNET; 64761; - EuPathDB; HostDB:ENSG00000059378.12; - GeneCards; PARP12; - HGNC; HGNC:21919; PARP12 HPA; HPA003584; - HPA; HPA063872; - MIM; 612481; gene neXtProt; NX_Q9H0J9; - OpenTargets; ENSG00000059378; - PharmGKB; PA134953063; - eggNOG; ENOG410IEI4; Eukaryota eggNOG; ENOG410ZFB8; LUCA GeneTree; ENSGT00760000119084; - HOGENOM; HOG000236279; - HOVERGEN; HBG050384; - InParanoid; Q9H0J9; - KO; K15259; - OMA; IKLHICQ; - OrthoDB; EOG091G14UY; - PhylomeDB; Q9H0J9; - TreeFam; TF338389; - ChiTaRS; PARP12; human EvolutionaryTrace; Q9H0J9; - GeneWiki; PARP12; - GenomeRNAi; 64761; - PRO; PR:Q9H0J9; - Proteomes; UP000005640; Chromosome 7 Bgee; ENSG00000059378; - CleanEx; HS_PARP12; - ExpressionAtlas; Q9H0J9; baseline and differential Genevisible; Q9H0J9; HS GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW GO; GO:0003950; F:NAD+ ADP-ribosyltransferase activity; IEA:UniProtKB-EC GO; GO:0003723; F:RNA binding; HDA:UniProtKB Gene3D; 1.10.10.10; -; 1 Gene3D; 3.30.720.50; -; 1 InterPro; IPR012317; Poly(ADP-ribose)pol_cat_dom InterPro; IPR036388; WH-like_DNA-bd_sf InterPro; IPR004170; WWE-dom InterPro; IPR037197; WWE_dom_sf InterPro; IPR000571; Znf_CCCH Pfam; PF00644; PARP; 1 Pfam; PF02825; WWE; 1 Pfam; PF00642; zf-CCCH; 1 SMART; SM00356; ZnF_C3H1; 3 SUPFAM; SSF117839; SSF117839; 1 PROSITE; PS51059; PARP_CATALYTIC; 1 PROSITE; PS50918; WWE; 2 PROSITE; PS50103; ZF_C3H1; 4 1: Evidence at protein level; 3D-structure; Complete proteome; Metal-binding; NAD; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transferase; Zinc; Zinc-finger CHAIN 1 701 Poly [ADP-ribose] polymerase 12 /FTId=PRO_0000211341 DOMAIN 298 361 WWE 1. {ECO:0000255|PROSITE- ProRule:PRU00248} DOMAIN 364 458 WWE 2. {ECO:0000255|PROSITE- ProRule:PRU00248} DOMAIN 484 698 PARP catalytic. {ECO:0000255|PROSITE- ProRule:PRU00397} ZN_FING 94 119 C3H1-type 1. {ECO:0000255|PROSITE- ProRule:PRU00723} ZN_FING 150 179 C3H1-type 2. {ECO:0000255|PROSITE- ProRule:PRU00723} ZN_FING 180 202 C3H1-type 3. {ECO:0000255|PROSITE- ProRule:PRU00723} ZN_FING 270 297 C3H1-type 4. {ECO:0000255|PROSITE- ProRule:PRU00723} ZN_FING 271 296 C3H1-type 3. {ECO:0000255|PROSITE- ProRule:PRU00723} COMPBIAS 58 61 Poly-Ala MOD_RES 258 258 Phosphoserine VARIANT 293 293 V -> I (in dbSNP:rs34111764) /FTId=VAR_050463 VARIANT 463 463 V -> M (in dbSNP:rs35456446) /FTId=VAR_050464 VARIANT 620 620 A -> V (in dbSNP:rs17161356) /FTId=VAR_050465 CONFLICT 444 450 VQKNLVY -> MGGFGQH (in Ref. 4) CONFLICT 462 462 Y -> C (in Ref. 4; BAB15457) STRAND 499 502 {ECO:0000244|PDB:2PQF} HELIX 508 518 {ECO:0000244|PDB:2PQF} STRAND 524 533 {ECO:0000244|PDB:2PQF} HELIX 535 550 {ECO:0000244|PDB:2PQF} TURN 551 554 {ECO:0000244|PDB:2PQF} STRAND 559 565 {ECO:0000244|PDB:2PQF} HELIX 568 570 {ECO:0000244|PDB:2PQF} HELIX 571 577 {ECO:0000244|PDB:2PQF} TURN 581 584 {ECO:0000244|PDB:2PQF} STRAND 593 600 {ECO:0000244|PDB:2PQF} HELIX 601 605 {ECO:0000244|PDB:2PQF} STRAND 611 623 {ECO:0000244|PDB:2PQF} STRAND 626 629 {ECO:0000244|PDB:2PQF} STRAND 649 653 {ECO:0000244|PDB:2PQF} STRAND 655 657 {ECO:0000244|PDB:2PQF} STRAND 660 664 {ECO:0000244|PDB:2PQF} HELIX 666 668 {ECO:0000244|PDB:2PQF} STRAND 669 679 {ECO:0000244|PDB:2PQF} SEQUENCE 701 AA; 79064 MW; 35152182F24CD215 CRC64; MAQAGVVGEV TQVLCAAGGA LELPELRRRL RMGLSADALE RLLRQRGRFV VAVRAGGAAA APERVVLAAS PLRLCRAHQG SKPGCVGLCA QLHLCRFMVY GACKFLRAGK NCRNSHSLTT EHNLSVLRTH GVDHLSYNEL CQLLFQNDPW LLPEICQHYN KGDGPHGSCA FQKQCIKLHI CQYFLQGECK FGTSCKRSHD FSNSENLEKL EKLGMSSDLV SRLPTIYRNA HDIKNKSSAP SRVPPLFVPQ GTSERKDSSG SVSPNTLSQE EGDQICLYHI RKSCSFQDKC HRVHFHLPYR WQFLDRGKWE DLDNMELIEE AYCNPKIERI LCSESASTFH SHCLNFNAMT YGATQARRLS TASSVTKPPH FILTTDWIWY WSDEFGSWQE YGRQGTVHPV TTVSSSDVEK AYLAYCTPGS DGQAATLKFQ AGKHNYELDF KAFVQKNLVY GTTKKVCRRP KYVSPQDVTT MQTCNTKFPG PKSIPDYWDS SALPDPGFQK ITLSSSSEEY QKVWNLFNRT LPFYFVQKIE RVQNLALWEV YQWQKGQMQK QNGGKAVDER QLFHGTSAIF VDAICQQNFD WRVCGVHGTS YGKGSYFARD AAYSHHYSKS DTQTHTMFLA RVLVGEFVRG NASFVRPPAK EGWSNAFYDS CVNSVSDPSI FVIFEKHQVY PEYVIQYTTS SKPSVTPSIL LALGSLFSSR Q
Citations (0)

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References

Katoh,M. (2003) Int. J. Oncol. 23 (2), 541-547 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

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$ 295.00
Cat# AI10592
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