|Application ||WB, IHC|
|Other Accession||NM_030912, NP_112174|
|Reactivity||Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Horse, Bovine, Dog|
|Predicted||Human, Mouse, Rabbit, Zebrafish, Pig, Horse, Bovine, Dog|
|Alias Symbol||GERP, RNF27|
|Other Names||Probable E3 ubiquitin-protein ligase TRIM8, 6.3.2.-, Glioblastoma-expressed RING finger protein, RING finger protein 27, Tripartite motif-containing protein 8, TRIM8, GERP, RNF27|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-TRIM8 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||TRIM8 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase which plays different roles in immune pathways. Participates in the activation of interferon- gamma signaling by promoting proteasomal degradation of the repressor SOCS1 (PubMed:12163497). Plays a positive role in the TNFalpha and IL-1beta signaling pathways. Mechanistically, induces the 'lys-63' polyubiquitination of MAP3K7/TAK1 component leading to the activation of NF-kappa-B (PubMed:22084099, PubMed:23152791). Modulates also STAT3 activity through negative regulation of PIAS3, either by degradation of PIAS3 through the ubiquitin-proteasome pathway or exclusion of PIAS3 from the nucleus (PubMed:20516148).|
|Cellular Location||Cytoplasm. Nucleus. Note=Nucleo-cytoplasmic translocation is involved in regulation of NF-kappa-B|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Carinci,F., (2007) Transl Res 150 (4), 233-245 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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