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Mbd3 antibody - N-terminal region

Rabbit Polyclonal Antibody

     
  • WB - Mbd3 antibody - N-terminal region AI11692

    WB Suggested Anti-Mbd3 Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:1562500
    Positive Control: Mouse Heart
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9Z2D8
Other Accession NM_013595, NP_038623
Reactivity Human, Mouse, Rat, Dog
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 32kDa
Additional Information
Alias Symbol AI181826, AU019209
Other Names Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3, Mbd3
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-Mbd3 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsMbd3 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Mbd3
Function Acts as transcriptional repressor and plays a role in gene silencing. Does not bind DNA by itself. Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (By similarity). Recruits histone deacetylases and DNA methyltransferases.
Cellular Location Nucleus. Chromosome Note=Detected on chromatin, at promoter regions of active genes (By similarity). Nuclear, in discrete foci.
Tissue Location Highly expressed in brain, heart, kidney, liver, lung, skeletal muscle, spleen and testis. Detected at lower levels in embryonic stem cells. EMBL; AF072248; AAC68877.1; -; mRNA EMBL; AF120995; AAD48909.1; -; Genomic_DNA EMBL; BC038264; AAH38264.1; -; mRNA CCDS; CCDS24020.1; -. [Q9Z2D8-1] CCDS; CCDS78860.1; -. [Q9Z2D8-2] RefSeq; NP_001293072.1; NM_001306143.1. [Q9Z2D8-2] RefSeq; NP_038623.1; NM_013595.3. [Q9Z2D8-1] UniGene; Mm.7142; - ProteinModelPortal; Q9Z2D8; - SMR; Q9Z2D8; - BioGrid; 201332; 17 ComplexPortal; CPX-954; MBD3/NuRD nucleosome remodeling and deacetylase complex CORUM; Q9Z2D8; - DIP; DIP-46518N; - IntAct; Q9Z2D8; 13 MINT; Q9Z2D8; - STRING; 10090.ENSMUSP00000089948; - iPTMnet; Q9Z2D8; - PhosphoSitePlus; Q9Z2D8; - jPOST; Q9Z2D8; - PaxDb; Q9Z2D8; - PeptideAtlas; Q9Z2D8; - PRIDE; Q9Z2D8; - Ensembl; ENSMUST00000092295; ENSMUSP00000089948; ENSMUSG00000035478. [Q9Z2D8-1] Ensembl; ENSMUST00000105349; ENSMUSP00000100986; ENSMUSG00000035478. [Q9Z2D8-2] GeneID; 17192; - KEGG; mmu:17192; - UCSC; uc007gda.1; mouse. [Q9Z2D8-2] UCSC; uc007gdb.1; mouse. [Q9Z2D8-1] CTD; 53615; - MGI; MGI:1333812; Mbd3 eggNOG; KOG4161; Eukaryota eggNOG; ENOG4111HPQ; LUCA GeneTree; ENSGT00940000154013; - HOGENOM; HOG000013073; - HOVERGEN; HBG052417; - InParanoid; Q9Z2D8; - KO; K11591; - OMA; RQRMRYD; - OrthoDB; 1431783at2759; - PhylomeDB; Q9Z2D8; - TreeFam; TF325032; - Reactome; R-MMU-3214815; HDACs deacetylate histones Reactome; R-MMU-6804758; Regulation of TP53 Activity through Acetylation Reactome; R-MMU-73762; RNA Polymerase I Transcription Initiation Reactome; R-MMU-8943724; Regulation of PTEN gene transcription ChiTaRS; Mbd3; mouse PRO; PR:Q9Z2D8; - Proteomes; UP000000589; Chromosome 10 Bgee; ENSMUSG00000035478; Expressed in 283 organ(s), highest expression level in tracheobronchial tree ExpressionAtlas; Q9Z2D8; baseline and differential Genevisible; Q9Z2D8; MM GO; GO:0000785; C:chromatin; IDA:MGI GO; GO:0005737; C:cytoplasm; IDA:MGI GO; GO:0000792; C:heterochromatin; IDA:MGI GO; GO:0005654; C:nucleoplasm; TAS:Reactome GO; GO:0005634; C:nucleus; IDA:MGI GO; GO:0016581; C:NuRD complex; IPI:MGI GO; GO:0032991; C:protein-containing complex; IDA:MGI GO; GO:0003682; F:chromatin binding; IDA:MGI GO; GO:0003677; F:DNA binding; IDA:MGI GO; GO:0008327; F:methyl-CpG binding; ISS:UniProtKB GO; GO:0007568; P:aging; IEA:Ensembl GO; GO:0007420; P:brain development; IEA:Ensembl GO; GO:0048568; P:embryonic organ development; IEA:Ensembl GO; GO:0007507; P:heart development; IEA:Ensembl GO; GO:0016573; P:histone acetylation; IMP:MGI GO; GO:0001701; P:in utero embryonic development; IMP:MGI GO; GO:0006346; P:methylation-dependent chromatin silencing; IDA:MGI GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:MGI GO; GO:0044030; P:regulation of DNA methylation; IEA:Ensembl GO; GO:0032355; P:response to estradiol; IEA:Ensembl GO; GO:0031667; P:response to nutrient levels; IEA:Ensembl GO; GO:0009888; P:tissue development; IMP:MGI InterPro; IPR016177; DNA-bd_dom_sf InterPro; IPR032343; MBD2/MBD3_p55-bd InterPro; IPR037965; MBD3 InterPro; IPR025884; MeCpG-bd_2/3_C_dom InterPro; IPR001739; Methyl_CpG_DNA-bd PANTHER; PTHR12396:SF12; PTHR12396:SF12; 1 Pfam; PF01429; MBD; 1 Pfam; PF14048; MBD_C; 1 Pfam; PF16564; MBDa; 1 SMART; SM00391; MBD; 1 SUPFAM; SSF54171; SSF54171; 1 PROSITE; PS50982; MBD; 1 1: Evidence at protein level; Alternative splicing; Chromosome; Coiled coil; Complete proteome; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Ubl conjugation CHAIN 1 285 Methyl-CpG-binding domain protein 3 /FTId=PRO_0000096263 DOMAIN 1 69 MBD. {ECO:0000255|PROSITE- ProRule:PRU00338} COILED 221 279 COMPBIAS 229 283 Glu-rich MOD_RES 56 56 Phosphoserine MOD_RES 85 85 Phosphoserine {ECO:0000250|UniProtKB:O95983} MOD_RES 144 144 Phosphoserine {ECO:0000250|UniProtKB:O95983} CROSSLNK 73 73 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) {ECO:0000250|UniProtKB:O95983} CROSSLNK 90 90 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) {ECO:0000250|UniProtKB:O95983} CROSSLNK 92 92 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) {ECO:0000250|UniProtKB:O95983} VAR_SEQ 5 36 Missing (in isoform 2) /FTId=VSP_011082 SEQUENCE 285 AA; 32168 MW; E4E57BD48463643F CRC64; MERKRWECPA LPQGWEREEV PRRSGLSAGH RDVFYYSPSG KKFRSKPQLA RYLGGSMDLS TFDFRTGKML MNKMNKSRQR VRYDSSNQVK GKPDLNTALP VRQTASIFKQ PVTKITNHPS NKVKSDPQKA VDQPRQLFWE KKLSGLSAFD IAEELVRTMD LPKGLQGVGP GCTDETLLSA IASALHTSTL PITGQLSAAV EKNPGVWLNT AQPLCKAFMV TDDDIRKQEE LVQQVRKRLE EALMADMLAH VEELARDGEA PLDKACAEEE EEEEEEEEEP EPERV
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$ 295.00
Cat# AI11692
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