|Other Accession||NM_017819, NP_060289|
|Reactivity||Human, Mouse, Rat, Rabbit, Zebrafish, Horse, Bovine, Dog|
|Predicted||Human, Rabbit, Zebrafish|
|Calculated MW||47 KDa|
|Alias Symbol||FLJ20432, MRPP1, RG9MTD1|
|Other Names||Mitochondrial ribonuclease P protein 1, Mitochondrial RNase P protein 1, 2.1.1.-, HBV pre-S2 trans-regulated protein 2, RNA (guanine-9-)-methyltransferase domain-containing protein 1, Renal carcinoma antigen NY-REN-49, tRNA methyltransferase 10 homolog C, TRMT10C, MRPP1, RG9MTD1|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-RG9MTD1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||RG9MTD1 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mitochondrial tRNA N(1)-methyltransferase involved in mitochondrial tRNA maturation (PubMed:18984158, PubMed:21593607, PubMed:23042678, PubMed:27132592). Component of mitochondrial ribonuclease P, a complex composed of TRMT10C/MRPP1, HSD17B10/MRPP2 and MRPP3, which cleaves tRNA molecules in their 5'-ends (PubMed:18984158). Together with HSD17B10/MRPP2, forms a subcomplex of the mitochondrial ribonuclease P, named MRPP1-MRPP2 subcomplex, which displays functions that are independent of the ribonuclease P activity (PubMed:23042678, PubMed:29040705). The MRPP1-MRPP2 subcomplex catalyzes the formation of N(1)- methylguanine and N(1)-methyladenine at position 9 (m1G9 and m1A9, respectively) in tRNAs; TRMT10C/MRPP1 acting as the catalytic N(1)-methyltransferase subunit (PubMed:23042678). The MRPP1-MRPP2 subcomplex also acts as a tRNA maturation platform: following 5'- end cleavage by the mitochondrial ribonuclease P complex, the MRPP1-MRPP2 subcomplex enhances the efficiency of 3'-processing catalyzed by ELAC2, retains the tRNA product after ELAC2 processing and presents the nascent tRNA to the mitochondrial CCA tRNA nucleotidyltransferase TRNT1 enzyme (PubMed:29040705). In addition to tRNA N(1)-methyltransferase activity, TRMT10C/MRPP1 also acts as a mRNA N(1)-methyltransferase by mediating methylation of adenosine residues at the N(1) position of MT-ND5 mRNA (PubMed:29072297).|
|Cellular Location||Mitochondrion matrix, mitochondrion nucleoid|
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