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FBXL3 antibody - middle region

Rabbit Polyclonal Antibody

     
  • WB - FBXL3 antibody - middle region AI12217

    WB Suggested Anti-FBXL3 Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:312500
    Positive Control: Human Liver
  • SPECIFICATION
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9UKT7
Other Accession NM_012158, NP_036290
Reactivity Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Sheep, Horse, Yeast, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Chicken, Sheep, Horse, Bovine, Guinea Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 47kDa
Additional Information
Alias Symbol FBL3, FBL3A, FBXL3A
Other Names F-box/LRR-repeat protein 3, F-box and leucine-rich repeat protein 3A, F-box/LRR-repeat protein 3A, FBXL3, FBL3A, FBXL3A
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-FBXL3 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsFBXL3 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FBXL3
Synonyms FBL3A, FBXL3A
Function Substrate-recognition component of the SCF(FBXL3) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation (PubMed:17463251, PubMed:23452855, PubMed:27565346). The SCF(FBXL3) complex mainly acts in the nucleus and mediates ubiquitination and subsequent degradation of CRY1 and CRY2 (PubMed:17463251, PubMed:23452855, PubMed:27565346). Activity of the SCF(FBXL3) complex is counteracted by the SCF(FBXL21) complex (PubMed:23452855).
Cellular Location Nucleus. Cytoplasm. Note=Predominantly nuclear
Tissue Location Widely expressed. EMBL; AF129532; AAF04466.1; -; mRNA EMBL; AF126028; AAF37383.1; -; mRNA EMBL; AK314442; BAG37051.1; -; mRNA EMBL; BC072448; AAH72448.1; -; mRNA CCDS; CCDS9457.1; - RefSeq; NP_036290.1; NM_012158.2 RefSeq; XP_005266393.1; XM_005266336.1 UniGene; Hs.508284; - PDB; 4I6J; X-ray; 2.70 A; B=1-428 PDBsum; 4I6J; - ProteinModelPortal; Q9UKT7; - SMR; Q9UKT7; - BioGrid; 117615; 16 CORUM; Q9UKT7; - DIP; DIP-40770N; - IntAct; Q9UKT7; 10 MINT; Q9UKT7; - STRING; 9606.ENSP00000347834; - iPTMnet; Q9UKT7; - PhosphoSitePlus; Q9UKT7; - BioMuta; FBXL3; - DMDM; 37537866; - EPD; Q9UKT7; - MaxQB; Q9UKT7; - PaxDb; Q9UKT7; - PeptideAtlas; Q9UKT7; - PRIDE; Q9UKT7; - ProteomicsDB; 84852; - DNASU; 26224; - Ensembl; ENST00000355619; ENSP00000347834; ENSG00000005812 GeneID; 26224; - KEGG; hsa:26224; - UCSC; uc001vkd.4; human CTD; 26224; - DisGeNET; 26224; - EuPathDB; HostDB:ENSG00000005812.10; - GeneCards; FBXL3; - HGNC; HGNC:13599; FBXL3 HPA; HPA053283; - HPA; HPA065626; - MIM; 605653; gene neXtProt; NX_Q9UKT7; - OpenTargets; ENSG00000005812; - PharmGKB; PA28022; - eggNOG; ENOG410IT1T; Eukaryota eggNOG; ENOG410XT8H; LUCA GeneTree; ENSGT00390000007432; - HOGENOM; HOG000060185; - HOVERGEN; HBG051587; - InParanoid; Q9UKT7; - KO; K10269; - OMA; SWDAFIK; - OrthoDB; EOG091G0R67; - PhylomeDB; Q9UKT7; - TreeFam; TF352583; - Reactome; R-HSA-390471; Association of TriC/CCT with target proteins during biosynthesis Reactome; R-HSA-400253; Circadian Clock Reactome; R-HSA-8951664; Neddylation Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation UniPathway; UPA00143; - ChiTaRS; FBXL3; human GeneWiki; FBXL3; - GenomeRNAi; 26224; - PRO; PR:Q9UKT7; - Proteomes; UP000005640; Chromosome 13 Bgee; ENSG00000005812; - CleanEx; HS_FBXL3; - ExpressionAtlas; Q9UKT7; baseline and differential Genevisible; Q9UKT7; HS GO; GO:0005829; C:cytosol; ISS:UniProtKB GO; GO:0016604; C:nuclear body; IDA:HPA GO; GO:0005634; C:nucleus; ISS:UniProtKB GO; GO:0019005; C:SCF ubiquitin ligase complex; IDA:UniProtKB GO; GO:0000151; C:ubiquitin ligase complex; NAS:UniProtKB GO; GO:0004842; F:ubiquitin-protein transferase activity; EXP:Reactome GO; GO:0043153; P:entrainment of circadian clock by photoperiod; ISS:UniProtKB GO; GO:0043687; P:post-translational protein modification; TAS:Reactome GO; GO:0031648; P:protein destabilization; ISS:UniProtKB GO; GO:0000209; P:protein polyubiquitination; TAS:Reactome GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB GO; GO:0042752; P:regulation of circadian rhythm; IMP:UniProtKB GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB Gene3D; 3.80.10.10; -; 1 InterPro; IPR036047; F-box-like_dom_sf InterPro; IPR001810; F-box_dom InterPro; IPR032675; LRR_dom_sf Pfam; PF12937; F-box-like; 1 SMART; SM00256; FBOX; 1 SUPFAM; SSF81383; SSF81383; 1 1: Evidence at protein level; 3D-structure; Biological rhythms; Complete proteome; Cytoplasm; Leucine-rich repeat; Nucleus; Reference proteome; Repeat; Ubl conjugation pathway CHAIN 1 428 F-box/LRR-repeat protein 3 /FTId=PRO_0000119842 DOMAIN 34 81 F-box REPEAT 119 146 LRR 1 REPEAT 181 207 LRR 2 REPEAT 208 233 LRR 3 REPEAT 234 259 LRR 4 REPEAT 316 341 LRR 5 REPEAT 343 368 LRR 6 REPEAT 369 394 LRR 7 MUTAGEN 358 358 C->A: Loss of binding with CRY1 MUTAGEN 358 358 C->S: Decrease in binding efficiency with CRY2 and of CRY2 ubiquitination efficiency, loss of binding with CRY1 CONFLICT 10 10 R -> H (in Ref. 2; AAF37383) CONFLICT 13 13 S -> A (in Ref. 2; AAF37383) CONFLICT 353 353 R -> A (in Ref. 2; AAF37383) CONFLICT 364 364 I -> V (in Ref. 3; BAG37051) CONFLICT 412 412 V -> A (in Ref. 3; BAG37051) HELIX 42 48 {ECO:0000244|PDB:4I6J} HELIX 53 59 {ECO:0000244|PDB:4I6J} HELIX 64 67 {ECO:0000244|PDB:4I6J} TURN 68 71 {ECO:0000244|PDB:4I6J} HELIX 73 76 {ECO:0000244|PDB:4I6J} STRAND 77 83 {ECO:0000244|PDB:4I6J} TURN 86 88 {ECO:0000244|PDB:4I6J} STRAND 89 91 {ECO:0000244|PDB:4I6J} HELIX 96 106 {ECO:0000244|PDB:4I6J} TURN 107 109 {ECO:0000244|PDB:4I6J} STRAND 112 117 {ECO:0000244|PDB:4I6J} HELIX 121 131 {ECO:0000244|PDB:4I6J} STRAND 140 149 {ECO:0000244|PDB:4I6J} HELIX 151 154 {ECO:0000244|PDB:4I6J} HELIX 157 170 {ECO:0000244|PDB:4I6J} STRAND 176 178 {ECO:0000244|PDB:4I6J} HELIX 186 196 {ECO:0000244|PDB:4I6J} TURN 197 199 {ECO:0000244|PDB:4I6J} STRAND 202 204 {ECO:0000244|PDB:4I6J} HELIX 213 222 {ECO:0000244|PDB:4I6J} STRAND 228 232 {ECO:0000244|PDB:4I6J} HELIX 233 235 {ECO:0000244|PDB:4I6J} HELIX 238 245 {ECO:0000244|PDB:4I6J} STRAND 246 249 {ECO:0000244|PDB:4I6J} STRAND 253 260 {ECO:0000244|PDB:4I6J} HELIX 274 283 {ECO:0000244|PDB:4I6J} STRAND 288 294 {ECO:0000244|PDB:4I6J} STRAND 303 306 {ECO:0000244|PDB:4I6J} STRAND 312 317 {ECO:0000244|PDB:4I6J} HELIX 321 328 {ECO:0000244|PDB:4I6J} STRAND 335 340 {ECO:0000244|PDB:4I6J} HELIX 348 357 {ECO:0000244|PDB:4I6J} STRAND 363 366 {ECO:0000244|PDB:4I6J} HELIX 373 382 {ECO:0000244|PDB:4I6J} HELIX 384 386 {ECO:0000244|PDB:4I6J} STRAND 388 393 {ECO:0000244|PDB:4I6J} HELIX 394 396 {ECO:0000244|PDB:4I6J} HELIX 405 416 {ECO:0000244|PDB:4I6J} SEQUENCE 428 AA; 48707 MW; AAEAB31BAF0EA5A5 CRC64; MKRGGRDSDR NSSEEGTAEK SKKLRTTNEH SQTCDWGNLL QDIILQVFKY LPLLDRAHAS QVCRNWNQVF HMPDLWRCFE FELNQPATSY LKATHPELIK QIIKRHSNHL QYVSFKVDSS KESAEAACDI LSQLVNCSLK TLGLISTARP SFMDLPKSHF ISALTVVFVN SKSLSSLKID DTPVDDPSLK VLVANNSDTL KLLKMSSCPH VSPAGILCVA DQCHGLRELA LNYHLLSDEL LLALSSEKHV RLEHLRIDVV SENPGQTHFH TIQKSSWDAF IRHSPKVNLV MYFFLYEEEF DPFFRYEIPA THLYFGRSVS KDVLGRVGMT CPRLVELVVC ANGLRPLDEE LIRIAERCKN LSAIGLGECE VSCSAFVEFV KMCGGRLSQL SIMEEVLIPD QKYSLEQIHW EVSKHLGRVW FPDMMPTW
Research Areas
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References

Busino,L.,(2007)Science316(5826),900-904ReconstitutionandStorage:Forshorttermuse,storeat2-8Cupto1week.Forlongtermstorage,storeat-20Cinsmallaliquotstopreventfreeze-thawcycles.

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$ 295.00
Cat# AI12217
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