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WBP11 antibody - N-terminal region

Rabbit Polyclonal Antibody

  • IHC - WBP11 antibody - N-terminal region AI12665
    Rabbit Anti-WBP11 Antibody
    Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue Observed Staining: Cytoplasmic in cell bodies of pinealocytes and their processes
    Primary Antibody
    Concentration: 1:100 Other Working Concentrations: 1/600
    Secondary Antibody: Donkey anti-Rabbit-Cy3
    Secondary Antibody
    Concentration: 1:200
    Magnification: 20X
    Exposure Time: 0.5 - 2.0 sec
  • IP - WBP11 antibody - N-terminal region AI12665
    Amount and Sample Type : 500 ug mouse brain homogenate Amount of IP Antibody : 6 ug Primary Antibody : WBP11
    Primary Antibody Dilution : 1:500
    Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594 Secondary Antibody Dilution : 1:5000
    Gene Name : WBP11 Submitted by : Dr. Yuzhi Chen, University of Arkansas for Medical Science
  • WB - WBP11 antibody - N-terminal region AI12665

    WB Suggested Anti-WBP11 Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:12500
    Positive Control: Hela cell lysate
    WBP11 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells
  • WB - WBP11 antibody - N-terminal region AI12665
    Lanes: 1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)
    Primary Antibody Dilution: 2μg/ml
    Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
    Secondary Antibody Dilution: 1: 20,000
    Gene Name: WBP11
    Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
  • WB - WBP11 antibody - N-terminal region AI12665

    Host: Rabbit
    Target Name: WBP11
    Sample Tissue: Jurkat
    Antibody Dilution: 1.0μg/mlWBP11 is strongly supported by BioGPS gene expression data to be expressed in Jurkat
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y2W2
Other Accession NM_016312, NP_057396
Reactivity Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Horse, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Rabbit, Zebrafish, Horse, Guinea Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 70kDa
Additional Information
Alias Symbol DKFZp779M1063, NPWBP, SIPP1, WBP-11
Other Names WW domain-binding protein 11, WBP-11, Npw38-binding protein, NpwBP, SH3 domain-binding protein SNP70, Splicing factor that interacts with PQBP-1 and PP1, WBP11, NPWBP, SIPP1, SNP70
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-WBP11 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsWBP11 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WBP11
Synonyms NPWBP, SIPP1, SNP70
Function Activates pre-mRNA splicing. May inhibit PP1 phosphatase activity.
Cellular Location Nucleus. Cytoplasm. Note=Predominantly located in the nucleus with granular heterogeneous distribution Excluded from nucleoli in interphase cells, distributed throughout cytoplasm in dividing cells. Colocalized with SC35 and U2B in the nucleus. In the cytoplasm, associates with the intermediate filament protein vimentin
Tissue Location Ubiquitous. Highly expressed in the heart, pancreas, kidney skeletal muscle, placenta and brain (at protein level). Weakly expressed in liver and lung EMBL; AB029309; BAA88410.1; -; mRNA EMBL; AF118023; AAD30425.1; -; mRNA EMBL; BC001621; AAH01621.1; -; mRNA EMBL; BC016441; AAH16441.2; -; mRNA EMBL; BC023532; AAH23532.1; -; mRNA CCDS; CCDS8666.1; - RefSeq; NP_057396.1; NM_016312.2 UniGene; Hs.655138; - ProteinModelPortal; Q9Y2W2; - SMR; Q9Y2W2; - BioGrid; 119702; 55 CORUM; Q9Y2W2; - ELM; Q9Y2W2; - IntAct; Q9Y2W2; 41 MINT; Q9Y2W2; - STRING; 9606.ENSP00000261167; - iPTMnet; Q9Y2W2; - PhosphoSitePlus; Q9Y2W2; - BioMuta; WBP11; - DMDM; 74735242; - EPD; Q9Y2W2; - MaxQB; Q9Y2W2; - PaxDb; Q9Y2W2; - PeptideAtlas; Q9Y2W2; - PRIDE; Q9Y2W2; - ProteomicsDB; 85915; - DNASU; 51729; - Ensembl; ENST00000261167; ENSP00000261167; ENSG00000084463 GeneID; 51729; - KEGG; hsa:51729; - UCSC; uc001rci.4; human CTD; 51729; - EuPathDB; HostDB:ENSG00000084463.7; - GeneCards; WBP11; - HGNC; HGNC:16461; WBP11 HPA; HPA039710; - HPA; HPA040037; - HPA; HPA046403; - HPA; HPA049126; - neXtProt; NX_Q9Y2W2; - OpenTargets; ENSG00000084463; - PharmGKB; PA38144; - eggNOG; KOG4672; Eukaryota eggNOG; ENOG41115S5; LUCA GeneTree; ENSGT00730000111015; - HOGENOM; HOG000154840; - HOVERGEN; HBG056793; - InParanoid; Q9Y2W2; - KO; K12866; - OMA; NKKQRQM; - OrthoDB; EOG091G0BKC; - PhylomeDB; Q9Y2W2; - TreeFam; TF323226; - Reactome; R-HSA-72163; mRNA Splicing - Major Pathway ChiTaRS; WBP11; human GeneWiki; WBP11; - GenomeRNAi; 51729; - PRO; PR:Q9Y2W2; - Proteomes; UP000005640; Chromosome 12 Bgee; ENSG00000084463; - CleanEx; HS_WBP11; - ExpressionAtlas; Q9Y2W2; baseline and differential Genevisible; Q9Y2W2; HS GO; GO:0005829; C:cytosol; IDA:HPA GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA GO; GO:0016607; C:nuclear speck; IEA:Ensembl GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0005634; C:nucleus; TAS:ProtInc GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central GO; GO:0019888; F:protein phosphatase regulator activity; IEA:Ensembl GO; GO:0003723; F:RNA binding; HDA:UniProtKB GO; GO:0003697; F:single-stranded DNA binding; TAS:ProtInc GO; GO:0050699; F:WW domain binding; IPI:UniProtKB GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IBA:GO_Central GO; GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW InterPro; IPR019007; WW_dom-bd_prot_11 Pfam; PF09429; Wbp11; 1 1: Evidence at protein level; Acetylation; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Isopeptide bond; Methylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; rRNA processing; Ubl conjugation CHAIN 1 641 WW domain-binding protein 11 /FTId=PRO_0000065945 REGION 1 45 Required for nuclear import REGION 217 221 Interaction with PP1. REGION 306 310 Interaction with PP1. COILED 75 133 MOTIF 455 466 PGR COMPBIAS 140 209 Pro-rich COMPBIAS 246 297 Asp-rich COMPBIAS 319 322 Poly-Lys COMPBIAS 393 538 Pro-rich MOD_RES 13 13 N6-acetyllysine MOD_RES 181 181 Phosphoserine MOD_RES 192 192 Omega-N-methylarginine MOD_RES 236 236 Phosphotyrosine {ECO:0000250|UniProtKB:Q923D5} MOD_RES 237 237 Phosphoserine MOD_RES 279 279 Phosphoserine MOD_RES 283 283 Phosphoserine MOD_RES 353 353 Phosphoserine MOD_RES 361 361 Phosphoserine MOD_RES 364 364 Phosphoserine MOD_RES 565 565 N6-acetyllysine {ECO:0000250|UniProtKB:Q923D5} MOD_RES 600 600 Phosphoserine CROSSLNK 557 557 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) CROSSLNK 572 572 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) MUTAGEN 192 192 R->A: Loss of PQBP1-binding; when associated with A-197 and A-198 MUTAGEN 197 197 R->A: Loss of PQBP1-binding; when associated with A-192 and A-198 MUTAGEN 198 198 K->A: Loss of PQBP1-binding; when associated with A-192 and A-197 SEQUENCE 641 AA; 69998 MW; C94F9687D132CE77 CRC64; MGRRSTSSTK SGKFMNPTDQ ARKEARKREL KKNKKQRMMV RAAVLKMKDP KQIIRDMEKL DEMEFNPVQQ PQLNEKVLKD KRKKLRETFE RILRLYEKEN PDIYKELRKL EVEYEQKRAQ LSQYFDAVKN AQHVEVESIP LPDMPHAPSN ILIQDIPLPG AQPPSILKKT SAYGPPTRAV SILPLLGHGV PRLPPGRKPP GPPPGPPPPQ VVQMYGRKVG FALDLPPRRR DEDMLYSPEL AQRGHDDDVS STSEDDGYPE DMDQDKHDDS TDDSDTDKSD GESDGDEFVH RDNGERDNNE EKKSGLSVRF ADMPGKSRKK KKNMKELTPL QAMMLRMAGQ EIPEEGREVE EFSEDDDEDD SDDSEAEKQS QKQHKEESHS DGTSTASSQQ QAPPQSVPPS QIQAPPMPGP PPLGPPPAPP LRPPGPPTGL PPGPPPGAPP FLRPPGMPGL RGPLPRLLPP GPPPGRPPGP PPGPPPGLPP GPPPRGPPPR LPPPAPPGIP PPRPGMMRPP LVPPLGPAPP GLFPPAPLPN PGVLSAPPNL IQRPKADDTS AATIEKKATA TISAKPQITN PKAEITRFVP TALRVRRENK GATAAPQRKS EDDSAVPLAK AAPKSGPSVP VSVQTKDDVY EAFMKEMEGL L
Research Areas
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$ 295.00
Cat# AI12665
Availability: 2-3 days
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