|Application ||WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||29174 Da|
|Other Names||14-3-3 protein epsilon, 14-3-3E, YWHAE|
|Target/Specificity||A synthetic peptide corresponding to residues in human 14-3-3E was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||14-3-3E Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326).|
|Cellular Location||Nucleus. Cytoplasm. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.|
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Provided below are standard protocols that you may find useful for product applications.
The 14-3-3 epsilon protein (14-3-3E) is one of seven isoforms in the 14-3-3 protein family; a family which consists of acidic 30 kDa proteins expressed at high levels in neurons of the central nervous system (1). It is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3E binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (2). It also interacts with both, the insulin-like growth factor I receptor (IGFIR) and the insulin receptor substrate I (IRS-1), suggesting its role in the regulation of insulin sensitivity (3). 14-3-3E has also been implicated in the pathogenesis of small cell lung cancer (4).
1. Satoh J, et al. Am J Pathol. 165(2):577-92, 2004 2. The UniProt Consortium. The Universal Protein Resource (UniPro), Nucleic Acids Res. 36:D190-D195 (2008) 3. Craparo A, et al. J Biol Chem. 272(17):11663-9, 1997 4. NCBI Reference Sequence (RefSeq): a curated non- redundant sequence database of genomes, transcripts and proteins. Pruitt KD, Tatusova, T, Maglott DR Nucleic Acids Res 2007 Jan 1;35(Database issue):D61-5
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