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Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF |
|---|---|
| Primary Accession | P35611 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone Names | EP734Y |
| Calculated MW | 80955 Da |
| Gene ID | 118 |
| Other Names | Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA |
| Target/Specificity | A synthetic peptide corresponding to residues in the C-term of human Adducin was used as immunogen. |
| Dilution | WB~~1:500~1000 IHC~~1:250~500 |
| Format | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Adducin alpha Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ADD1 |
|---|---|
| Synonyms | ADDA |
| Function | Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. |
| Cellular Location | Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side |
| Tissue Location | Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; α and β, or α and γ subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha (5).
References
1. Gardner, K.; Bennett, V. : J. Biol. Chem. 261: 1339-1348, 1986.
2. Kuhlman, P.A., C.A. Hughes, V. Bennett, and V.M. Fowler. 1996. J. Biol. Chem. 271: 7986-7991
3. Dong, L., C. Chapline, B. Mousseau, L. Fowler, K. Ramsay, J.L. Stevens, and S. Jaken. 1995. J. Biol. Chem. 270: 25534-25540
4. Palfrey, H. C., Waseem, A. (1985) J. Biol. Chem. 260, 16021-16029
5. Fukata et al. J. Cell Biol., Volume 145, Number 2, April 19, 1999 347-361
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