|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||103854 Da|
|Other Names||Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human Alpha-Actinin 2 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Alpha-Actinin-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.|
|Cellular Location||Cytoplasm, myofibril, sarcomere, Z line Note=Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle|
|Tissue Location||Expressed in both skeletal and cardiac muscle.|
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Provided below are standard protocols that you may find useful for product applications.
Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibers, while ACTN3 expression is restricted to a subset of type 2 fibers (2).
1. Tsukada, S., et al (1993) Cell 72, 279-290
2. Rawlings, DJ et al. Immunol. 7, 237-246
3. Vassilev, AZ et al.. 1999. J. Biol. Chem. 274:1646-1656
4. Mahajan S, et al. Mol Cell Biol. 1995;15:5304
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