|Application ||WB, IHC|
|Calculated MW||311646 Da|
|Other Names||Adenomatous polyposis coli protein, Protein APC, Deleted in polyposis 25, APC, DP25|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human APC was used as immunogen. This antibody is predicted to detect isoform 2 based on sequence analysis.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||APC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.|
|Cellular Location||Cell junction, adherens junction. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle membrane Cytoplasm. Cell membrane. Note=Associated with the microtubule network at the growing distal tip of microtubules (PubMed:19632184). Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment (PubMed:19151759). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (PubMed:20937854).|
|Tissue Location||Expressed in a variety of tissues: brain, small intestine, colon, thymus, skeletal muscle, heart, prostate, lung, spleen, ovary, testis kidney, placenta, blood and liver (PubMed:21643010, PubMed:27217144). Isoform 1A: Very strongly expressed in brain but has relatively low expression levels in other tissues (PubMed:19527921, PubMed:21643010, PubMed:27217144) Isoform 1B: Predominant form in all tissues except for brain, including gastric mucosa and blood (PubMed:19527921, PubMed:21643010, PubMed:27217144).|
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Provided below are standard protocols that you may find useful for product applications.
Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling (1). APC regulates β-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration (2-3). Mutations cause the production of an APC protein that is abnormally short and nonfunctional. Defects in APC are a cause of familial aenomatous polyposis (FAP), Turcot syndrome, medulloblastoma (MDB), and other cancers. (4-6)
1. Senda T, Shimomura A, Iizuka-Kogo A. Anat Sci Int. 2005 Sep;80(3):121-31.
2. Narayan S, Roy D. Mol Cancer. 2003 Dec 12;2:41.
3. van Es JH, Giles RH, Clevers HC. Exp Cell Res. 2001 Mar 10;264(1):126-34.
4. Jarvinen HJ, Peltomaki P. Eur J Gastroenterol Hepatol. 2004 Jan;16(1):5-8.
5. Jaiswal AS, Balusu R, Narayan S. Front Biosci. 2005 May 1;10:1118-34.
6. Cohen MM Jr. Am J Med Genet A. 2003 Nov 1;122(4):303-14.
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