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Bcl-x Antibody

Rabbit Monoclonal Antibody

  • FC - Bcl-x Antibody AJ1084a
    C. Flow cytometric analysis of permeabilized K562 cells using anti-Bcl-x RabMAb (red) (Cat. #AJ1084a) or a Rabbit IgG (negative) (green).
  • IHC - Bcl-x Antibody AJ1084a
    B. Immunohistochemical analysis of human paraffin-embedded prostate carcinoma tissue using anti-Bcl-x RabMAb (Cat. #AJ1084a).
  • WB - Bcl-x Antibody AJ1084a
    A. Western blot analysis of K562 cell lysate using anti-Bcl-x RabMAb (Cat. #AJ1084a), 1:1,000 dilution.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q07817
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names E18
Calculated MW 26049 Da
Gene ID 598
Other Names Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, BCL2L1, BCL2L, BCLX
Target/Specificity A synthetic peptide corresponding to residues between BH3 and BH3 of Human Bcl-x was used as the immunogen. This antibody should recognize Bcl-xL, Bcl-xS and Bcl-x(beta) as the immunogen sequence is common to them. The antibody does not cross-react with other Bcl-2 family members.
Dilution FC~~1:50
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBcl-x Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL2L1
Synonyms BCL2L, BCLX
Function Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage- dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(S) promotes apoptosis.
Cellular Location Isoform Bcl-X(L): Mitochondrion inner membrane. Mitochondrion outer membrane. Mitochondrion matrix. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side. Note=After neuronal stimulation, translocates from cytosol to synaptic vesicle and mitochondrion membrane in a calmodulin-dependent manner (By similarity). Localizes to the centrosome when phosphorylated at Ser-49.
Tissue Location Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain
Research Areas
Citations (0)

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Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2 (2). It has been found that eterodimerization with Bax does not seem to be required for anti-apoptotic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis.


1. Boise, L.H. et al. Cell 74(4): 597-608 (1993)
2. Sedlak, T.W. et al. PNAS U S A. 92(17): 7834-8 (1995)
3. Cheng, E.H. et al. Nature 379(6565): 554-6 (1996)
4. Minn, A.J. et al. EMBO. J. 18: 632-643 (1999)

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Cat# AJ1084a
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