|Application ||WB, IHC|
|Other Names||BLNK, BASH, SLP65, B-cell linker protein, Cytoplasmic adapter protein;B-cell adapter containing a SH2 domain protein;B-cell adapter containing a Src homology 2 domain protein;Src homology 2 domain-containing leukocyte protein of 65 kDa|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-term of human BLNK was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BLNK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
B-cell linker protein (BLNK, also known as SLP65, BASH, and BCA) is a B cell adaptor that plays an important role in B cell development and in BCR-signal transduction. Since BLNK does not encode any intrinsic enzymatic activity, its function is to serve as a scaffold for assembling macromolecular complexes which includes enzymes (PLC gamma, Vav and Btk) and additional linker proteins (Grb2 and Nck) (1-3) . In addition, BLNK binds Btk and is required for activation of the transcription factor NF-κB. Tyrosine phosphorylation of BLNK by Syk provides specific docking sites for these SH2-containing effector proteins which in turn permits phosphorylation and/or activation of their respective signaling pathways.
1. Fu C., Turck,C., Kurosaki,T. and Chan,A. (1998) Immunity, 9, 93
2. Jumaa H., Mitterer,M., Reth,M. and Nielsen,P.J. (2001) Eur. J. Immunol., 31, 2164
3. Hashimoto S. et al. (1999). Blood, 94, 2357
4. Christopher W. Chiu, Mark Dalton, Masamichi Ishiai,Tomohiro Kurosaki,and Andrew C. Chan; EMBO J. 2002 December 2; 21(23): 6461
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.