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C/EBP Beta Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - C/EBP Beta Antibody (C-term) AJ1110a
    A. Western blot analysis on PC-12 cell lysate using anti-C/EBP Beta RabMAb (Cat. #AJ1110a), dilution 1:5,000.
  • IF - C/EBP Beta Antibody (C-term) AJ1110a
    B. Immunofluorescent staining of HeLa cells using anti-C/EBP Beta RabMAb (Cat. #AJ1110a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21272
Reactivity Human, Rat
Host Rabbit
Clonality Monoclonal
Clone Names E299
Calculated MW 31503 Da
Gene ID 24253
Other Names CCAAT/enhancer-binding protein beta, C/EBP beta, C/EBP-related protein 2, Interleukin-6-dependent-binding protein, IL-6DBP, Liver-enriched inhibitory protein, LIP, Liver-enriched transcriptional activator, LAP, Silencer factor B, SF-B, Cebpb, Crp2, Sfb
Target/Specificity A synthetic peptide corresponding to residues in C-terminus of rat C/EBP b was used as immunogen. The antibody can recognize three C/EBPb isoform (i.e. LAP*, LAP, and LIP).
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsC/EBP Beta Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Cebpb {ECO:0000312|RGD:2327}
Function Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:8336793). Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis (PubMed:10635333). The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA (By similarity). Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage (PubMed:10635333). Binds to regulatory regions of several acute- phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing (By similarity). During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation (By similarity). Essential for female reproduction because of a critical role in ovarian follicle development (By similarity). Restricts osteoclastogenesis: together with NFE2L1; represses expression of DSPP during odontoblast differentiation (PubMed:15308669).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P17676}. Cytoplasm {ECO:0000250|UniProtKB:P17676}. Note=Translocates to the nucleus when phosphorylated at Ser-288. In T-cells when sumoylated drawn to pericentric heterochromatin thereby allowing proliferation (By similarity). {ECO:0000250|UniProtKB:P17676, ECO:0000250|UniProtKB:P28033}
Tissue Location Liver and lung.
Citations ( 0 )


CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (α,β,γ,δ,ε, ζ ) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). A number of serine targets of PKA have been identified in C/EBP-β, suggesting its activities are post-translationally regulated by cAMP (2). Even though, it has been show that C/EBP β occurs predominantly as a heterodimer (3), C/EBP β & γ readily heterodimerize with each other as well as with C/EBP α (4). Additionally, 3 isoforms of C /EBP β can be detected (LAP*, LAP, & LIP) (5).


1. Julie Lekstrom-Himes and Kleanthis G. Xanthopoulos, J Biol Chem, Vol. 273, Issue 44, 28545-28548, October 30, 1998
2. Brasier AR, and Li J. Mechanisms for Inducible Control of Angiotensinogen Gene Transcription. Hypertension 27:465-475 (1996).
3. Sara E. Parkin , Mark Baer , Terry D. Copeland, Richard C. Schwartz , and Peter F. Johnson, J. Biol. Chem., Vol. 277, Issue 26, 23563-23572, June 28, 2002
4. H Thomassin, D Hamel, D Bernier, M Guertin and L Belanger. Nucleic Acids Research, Vol 20, Issue 12 3091-3098, 1992
5. Wen-Chi Su, et al. J. Biol. Chem., Vol. 278, Issue 51, 51150-51158, December 19, 2003

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Cat# AJ1110a
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