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Calpactin-I Antibody (light chain)

Rabbit Monoclonal Antibody

  • WB - Calpactin-I Antibody (light chain) AJ1120a
    A. Western blot analysis on (A) HaCaT, (B) A431 and (C) HT-29 cell lysates using anti-Calpactin I Light Chain/S100A10 RabMAb (Cat. #AJ1120a), dilution 1:10,000.
  • IHC - Calpactin-I Antibody (light chain) AJ1120a
    B.Immunohistochemical analysis of paraffin-embedded human colon using anti-Calpactin I light chain/ S100A10 RabMAb (Cat. #AJ1120a).
  • IHC - Calpactin-I Antibody (light chain) AJ1120a
    C.Immunohistochemical analysis of paraffin-embedded human kidneys using anti- Calpactin I light chain/ S100A10 RabMAb (Cat. #AJ1120a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P60903
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR3317
Calculated MW 11203 Da
Gene ID 6281
Other Names Protein S100-A10, Calpactin I light chain, Calpactin-1 light chain, Cellular ligand of annexin II, S100 calcium-binding protein A10, p10 protein, p11, S100A10, ANX2LG, CAL1L, CLP11
Target/Specificity A synthetic peptide corresponding to residues in human Calpactin I light chain/S100A10 was used as an immunogen.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCalpactin-I Antibody (light chain) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name S100A10
Synonyms ANX2LG, CAL1L, CLP11
Function Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine- specific kinase.
Research Areas
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S100 proteins are low-molecular-weight calcium-binding proteins of the EF-hand superfamily and appear to be involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation (1). The Calpactin I light chain (S100 calcium-binding protein A10; S100A10) is a member of the S100 family and it forms a heterotetrameric complex with another calcium-binding protein, annexin II(2). S100A10 is present in basal and spinous cells, in the cytoplasm, and is associated with the plasma membrane (3). The basic unit for S100A10 is a tight, non-covalent dimmer (4). S100A10, as well as a few other members of the S100 family, are suggested as transglutaminase substrates. Also, S100 proteins have been reported to localize to the plasma membrane in differentiated keratinocytes, suggesting a role in regulating calcium-dependent, membrane-associated events (3).


1. Schafer BW, et al. Genomics 25(3):638-43, 1995
2. Kube E, et al. Gene. 102(2):255-9, 1991
3. Broome AM, et al. J Histochem Cytochem 51(5):675-685, 2003
4. Rety S, et al. Nat Struct Biol. 6(1):89-95, 1999

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Cat# AJ1120a
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