|Calculated MW||18696 Da|
|Other Names||T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD247, CD3Z, T3Z, TCRZ|
|Target/Specificity||A synthetic peptide corresponding to residues in the C-terminus of human CD3 zeta was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD3-zeta Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CD3Z, T3Z, TCRZ|
|Function||Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083). CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083). Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs) (PubMed:11722641).|
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Provided below are standard protocols that you may find useful for product applications.
CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR) (1). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C (1,2). CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways (3). Loss of the zeta chain results in the synthesis of unstable TCRs (4). A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases (5).
1. Weiss, A., et al. Sem. Immunol. 3: 313
2. Siegel, J.N., et al. Sem Immunol. 3: 325
3. Irving, B. A., and Weiss, A. (1991) Cell 64, 891-901
4. Minami, Y., Weissman, A.M., Samelson, L.E., and Klausner, R.D. 1987.Natl. Acad. Sci. USA. 84:2688-2692
5. Zea, A. H., Curti, B. D., Longo, D. L., Alvord, W. G., Strobl, S. L., Mizoguchi, H., Creekmore, S. P., O'Shea, J. J., Powers, G. C., Urba, W. J., and Ochoa, A. C. (1995) Clin. Cancer Res. 1, 1327-1335
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