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CD45 Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - CD45 Antibody (C-term) AJ1164b
    A. Western blot analysis on Jurkat cell lysate using anti-CD45 RabMAb (Cat. #AJ1164b).
  • FC - CD45 Antibody (C-term) AJ1164b
    B. Flow cytometric analysis of permeabilized Jurkat cells using anti-CD45 RabMAb (red) (Cat. #AJ1164b) and a rabbit IgG (negative) (green).
  • IHC - CD45 Antibody (C-term) AJ1164b
    C. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-CD45 RabMAb (Cat. #AJ1164b)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08575
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP322Y
Calculated MW 147486 Da
Gene ID 5788
Other Names Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
Target/Specificity A synthetic peptide corresponding to residues in the C-terminal domain of human CD45 was used as immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD45 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTPRC (HGNC:9666)
Synonyms CD45
Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein Membrane raft. Note=Colocalized with DPP4 in membrane rafts
Research Areas
Citations (0)

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CD45, also known as the leukocyte common antigen (LCA), is a transmembrane protein-tyrosine phosphatase (PTPase) that is expressed in hematopoietic cells except erythrocytes and platelets (1). CD 45 has been implicated in activating, differentiating and the development of the immune system. It regulates T-or B-cell activation during receptor-specific recognition by dephosphorylating tyrosine residues in protein kinase substrates. It is also required for T- and B-cell activation by regulating Lck & Fyn and Lyn & Blk respectively (2). Additionally, the expression of the CD45 5 isoforms can be seen in the various stages of differentiation of normal hematopoietic cells depending on the activation status of the cell (3).


1. Braford, D., Flint, A.J., Tonks, N.K., Crystal structure of human phosphotase 1B. 1994. Science 11:253(5152):1373.
2. Kung, C., Pingel, J.T., Heikinheimo, M., Klemola, T., Varkila, K., Yoo, L.I., Vuopala, k., Poyhonen, M., Rogers, M., Speck, S.H., Chatilla, T.A., and T.M. Matthews. (2000) Nature-Medicine.
3. Virts, E., Barritt, D., Siden, E., and R.A., William. 1997. Immunology 34(16-17) 1119-1197

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Cat# AJ1164b
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