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Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P46109 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone Names | EP270Y |
| Calculated MW | 33777 Da |
| Gene ID | 1399 |
| Other Names | Crk-like protein, CRKL |
| Target/Specificity | A synthetic phospho-peptide corresponding to residues surrounding Tyr207 of human CrkL was used as immunogen. The antibody only detects CrkL phosphorylated on Tyrosine 207. |
| Dilution | WB~~1:1000~5000 |
| Format | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CrkL Antibody Phospho (pY207) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CRKL |
|---|---|
| Function | May mediate the transduction of intracellular signals. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CrkL (Crk-like), a 36kDa protein predominantly expressed in hematopoietic cell lines, is a member of the Crk adapter protein family. Lacking any intrinsic enzymatic activity, CrkL is composed of one SH2 domain and two SH3 domains (1). The SH2 domain of CrkL as been shown to interact with c-Cbl, Cas, Hef-1 and Paxillin (2); while the N-terminal SH3 can bind to c-ABL, Sos1 and C3G (3). Additionally, the phosphorylated form of CrkL (pY207) binds to BCr-ABL oncogene in chronic myelogenous leukemia cells (4).
References
1. Hoeve J, et al. Oncogene 8:2469-2474, 1993.
2. Kyono, W, et al. J. Immunol. 161:5555-5563, 1998.
3. Sattler, M, et al. J. Biol. Chem. 272:14320-14326, 1997.
4. Ling, P, et al. Mol. Cell. Biol. 19:1359-1368, 1999.
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