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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Cyclin D3 Antibody   

Cyclin D3 Antibody

Rabbit Monoclonal Antibody

  • WB - Cyclin D3 Antibody AJ1210a
    A. Western blot analysis on K562 cell lysate of using anti-Cyclin D3 Rabbit Mab (Cat. #AJ1210a), dilution 1:5000
  • IF - Cyclin D3 Antibody AJ1210a
    B. Immunofluorescent staining of HeLa cells using anti-Cyclin D3 RabMAb, (Cat. #AJ1210a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30281
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP463E
Calculated MW 32520 Da
Gene ID 896
Other Names G1/S-specific cyclin-D3, CCND3
Target/Specificity A synthetic peptide corresponding to a residue near the C-term of human Cyclin D3 was used as an immunogen.
Dilution WB~~1:20000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCyclin D3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCND3
Function Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Cellular Location Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members
Research Areas
Citations (0)

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Cyclin D3 is a member of Cyclin D family which mediates cell cycle progression through the G1 phase. Cyclin D family binds and activates cyclin-dependent kinases 4 and 6 (CDK4, CDK6), which regulates the phosphorylation of retinoblastoma (Rb) protein, p107, and p130 (1). Cyclin D3, similar to cyclin D1 and D2, regulates the initial G1 to S transition by inhibiting Rb and activating E2F proteins (2). Cyclin D3 expression has been linked to inhibition of granulocyte differentiation and activation of various transcription factors, such as transcription factor 5 (3, 4). Overexpression of cyclin D3 is associated with poor clinical outcome in diffuse large B-cell lymphoma cases (5).


1. Sarruf D, et al. MCB, 25(22):9985-9995, 1995.
2. Dyson N, et al. Genes Dev. 12:2245-2262, 1998.
3. Liu W, et al. Biochem. Biophys. Res. Commun. 321:954-960, 2004.
4. Kato JY, et al. PNAS 90:11513-11517, 1993.
5. Filipits M, et al. Clin. Can. Res. 8:729-733, 2002.

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Cat# AJ1210a
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