|Application ||WB, IHC, IF|
|Calculated MW||25565 Da|
|Other Names||Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II, MT-CO2, COII, COXII, MTCO2|
|Target/Specificity||A synthetic peptide corresponding to residues in human Cytochrome C Oxidase subunit 2 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Cytochrome-C Antibody (oxidase subunit 2) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.|
|Cellular Location||Mitochondrion inner membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Cytochrome c oxidase subunit 2 (CO2 or MTCO2) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It collects electrons from ferrocytochrome c (reduced cytochrome c) and transfers them to oxygen to give water. The energy released is used to transport protons across the mitochondrial inner membrane. Complex IV is composed of 13 polypeptides. Subunits 1, 2 and 3 are encoded by the mtDNA while the remaining subunits are nuclear encoded (1-3).
1. Kadenbach B, et al. Anal Biochem. 129(2):517-21, 1983
2. Capaldi RA., et al. Annu Rev Biochem.59:569-96, 1990
3. Shoffner, J.M., et al. The Metabolic and Molecular Basis of Inherited Disease 1(7):1535−1609
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