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Cytochrome-C Antibody (oxidase subunit 2)

Rabbit Monoclonal Antibody

  • WB - Cytochrome-C Antibody (oxidase subunit 2) AJ1216c
    A. Western blot analysis on (A) K562, (B) MCF-7, (C) THP-1 and (D) HeLa cell lysates using anti-Cytochrome C Oxidase subunit 2 RabMAb (Cat. #AJ1216c), dilution 1:10,000.
  • IHC - Cytochrome-C Antibody (oxidase subunit 2) AJ1216c
    C. . Immunohistochemical analysis of paraffin-embedded human heart muscles tissue using anti-Cytochrome C oxidase subunit 2 RabMAb (Cat. #AJ1216c).
  • IHC - Cytochrome-C Antibody (oxidase subunit 2) AJ1216c
    B. . Immunohistochemical analysis of paraffin-embedded human heart liver tissue using anti-Cytochrome C oxidase subunit 2 RabMAb (Cat. #AJ1216c).
  • IF - Cytochrome-C Antibody (oxidase subunit 2) AJ1216c
    D. Immunofluorescent staining of HeLa cells using anti-Cytochrome C oxidase subunit 2 RabMAb (Cat. #AJ1216c).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00403
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR3314
Calculated MW 25565 Da
Gene ID 4513
Other Names Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II, MT-CO2, COII, COXII, MTCO2
Target/Specificity A synthetic peptide corresponding to residues in human Cytochrome C Oxidase subunit 2 was used as an immunogen.
Dilution WB~~1:10000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCytochrome-C Antibody (oxidase subunit 2) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MT-CO2
Function Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Cellular Location Mitochondrion inner membrane; Multi-pass membrane protein
Research Areas
Citations (0)

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Cytochrome c oxidase subunit 2 (CO2 or MTCO2) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It collects electrons from ferrocytochrome c (reduced cytochrome c) and transfers them to oxygen to give water. The energy released is used to transport protons across the mitochondrial inner membrane. Complex IV is composed of 13 polypeptides. Subunits 1, 2 and 3 are encoded by the mtDNA while the remaining subunits are nuclear encoded (1-3).


1. Kadenbach B, et al. Anal Biochem. 129(2):517-21, 1983
2. Capaldi RA., et al. Annu Rev Biochem.59:569-96, 1990
3. Shoffner, J.M., et al. The Metabolic and Molecular Basis of Inherited Disease 1(7):1535−1609

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Cat# AJ1216c
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