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eEF-2K Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - eEF-2K Antibody (C-term) AJ1251b
    A. Western blot analysis on Hek293 cell lysate of using Anti-eEF2k (C-term) Rabbit Monoclonal (Cat. #AJ1251b), dilution 1:1000.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00418
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP881Y
Calculated MW 82144 Da
Gene ID 29904
Other Names Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K
Target/Specificity A synthetic peptide corresponding to residues in the C-terminal of human eEF2 was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionseEF-2K Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF2K
Function Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Research Areas
Citations (0)

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Eukaryotic elongation factor-2 kinase (eEF-2k) previously known as Ca2+/calmodulin-dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor-2 (eEf-2) (1). Phosphorylation of eEF-2 by eEF-2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation (2). The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively via phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK) (3-4). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogenesis and malignant tumors (5-6).


1. Ryazanov et al. Proc Nat Acad Sci 94: 4884-4889, 1997
2. Ryazanov AG, et al. Nature 334:170-173, 1988.
3. Diggle, TA, et al. FEBS Lett 457:189-192, 1999.
4. Wang, XW, EMBO J 20:4370-4379, 2001.
5. End D, et al. J Biol Chem 257:9223-9225, 1988.
6. Severinov, KV, et al. New Biol 2: 887-893, 1990.

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Cat# AJ1251b
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