|Reactivity||Human, Mouse, Rat|
|Calculated MW||94255 Da|
|Other Names||Epidermal growth factor receptor substrate 15-like 1, Eps15-related protein, Eps15R, EPS15L1, EPS15R|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human EPS15R was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EPS15R Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.|
|Cellular Location||Cell membrane; Peripheral membrane protein. Nucleus. Membrane, coated pit. Note=Localized to plasma membrane coated pits.|
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Provided below are standard protocols that you may find useful for product applications.
EPS15 and EPS15R are substrates of the epidermal growth factor (EGF) receptor kinase that are characterized by the presence of a protein:protein interaction domain, the EH domain, and by their ability to bind to the clathrin adaptor protein complex adaptor protein 2. Evidence suggests that eps15 and eps15R are involved in endocytosis (1). The most striking difference between these two related proteins is that Eps15R is also found in the nucleus, whereas Eps15 is excluded from this compartment at steady state (2). Eps15R plays a key role in clathrin-mediated endocytosis of transmembrane receptors. Using a mutational approach, it has been found that the second ubiquitin-interacting motif (UIM) of Eps15 and Eps15R is essential for their ubiquitination (3).
1. Carbone R, et al. Cancer Res., 57(24):5498-504, 1997.
2. Poupon V, et al. J Biol Chem, 277(11):8941-8, 2002.
3. Klapisz E, et al. J Biol Chem, 277(34):30746-53, 2002.
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