|Application ||WB, IHC|
|Calculated MW||77244 Da|
|Other Names||Amyloid beta A4 precursor protein-binding family B member 1, Protein Fe65, APBB1, FE65, RIR|
|Target/Specificity||A synthetic peptide corresponding to residues in human FE65 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FE65 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro- apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).|
|Cellular Location||Cell membrane. Cytoplasm. Nucleus. Cell projection, growth cone. Nucleus speckle Note=Colocalizes with TSHZ3 in axonal growth cone (By similarity) In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus. Colocalizes with NEK6 at the nuclear speckles. Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity).|
|Tissue Location||Highly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease|
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Provided below are standard protocols that you may find useful for product applications.
Amyloid beta A4 precursor protein-binding family B member 1 (FE65) is a member of a family of multidomain adaptor proteins (FE65, FE65L1, and FE65L2) that form multiprotein complexes with a range of functions. FE65 is highly expressed in the brain and it has three protein-protein interaction domains: a WW domain and two PID/PTB domains. Through the PTB2 domain, FE65 interacts with the Alzheimer?s disease amyloid precursor protein (APP) intracellular domain (AICD) and can alter APP processing. The WW domain is responsible for the interaction with various proteins, which in turn interacts with the cytoskeleton. The second PID/PTB domain of FE65 binds to the transcription factor, CP2/LSF/LBP1 protein (1, 2). FE65 also plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis (3).
1. McLoughlin DM, et al. J Neurosci Res. 86(4):744-54, 2008. 2. Russo, T. et al. FEBS Lett 434, 1-7, 1998. 3. The UniProt Consortium. The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2008).
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