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Hmgb3 Antibody

Rabbit Monoclonal Antibody

  • WB - Hmgb3 Antibody AJ1365a
    A. Western blot analysis on (A) 293T, (B) HeLa and (C) human placenta cell lysates using anti-Hmgb3 RabMAb (Cat. #AJ1365a), dilution 1:5000.
  • IHC - Hmgb3 Antibody AJ1365a
    B. Immunohistochemical analysis of paraffin-embedded human placenta using anti-Hmgb3 RabhMAb (Cat. #AJ1365a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15347
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP2839Y
Calculated MW 22980 Da
Gene ID 3149
Other Names High mobility group protein B3, High mobility group protein 2a, HMG-2a, High mobility group protein 4, HMG-4, HMGB3, HMG2A, HMG4
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human Hmgb3 was used as an immunogen.
Dilution WB~~1:2500~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHmgb3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HMGB3
Synonyms HMG2A, HMG4
Function Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non- canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P40618, ECO:0000255|PROSITE-ProRule:PRU00267}. Chromosome Cytoplasm {ECO:0000250|UniProtKB:O54879}
Tissue Location Expressed predominantly in placenta.
Citations ( 0 )


Hmgb3 belongs to the high mobility group (HMG) protein superfamily. It contains DNA-binding HMG box domains and is thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (1). Hmgb3 is a sequence-independent chromatin-binding protein that is preferentially expressed in hematopoietic stem cells (2). It can alter DNA structure to facilitate transcription factor binding. Enforced expression of Hmgb3 inhibits B-cell and myeloid differentiation and thus, down-regulation of Hmgb3 protein levels has been concluded to be an important step for myeloid and B-cell differentiation (3). Hmgb3 deficiency possibly leads to a failure of hematopoietic stem cells to expand into normal numbers of common lymphoid progenitors and common myeloid progenitors. Hmgb3 progenitors attain the ability to expand rapidly and differentiate into normal numbers of hematopoietic cells (4).


1. Wilke K, et al. Gene 198: 269-274, 1997
2. Nemeth MJ, et al. Proc Natl Acad Sci U S A 103(37):13783-8, 2006
3. Nemeth MJ, et al. Blood 102(4):1298-306, 2003
4. Nemeth MJ, et al. Blood 105 (2):627-34, 2005

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Cat# AJ1365a
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