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HSL Antibody Phospho (pS855)

Rabbit Monoclonal Antibody

  • WB - HSL Antibody Phospho (pS855) AJ1377a
    A. Western blot analysis on Human skeletal muscle lysates using anti-Phospho-HSL (pS855) RabMAb (Cat. #AJ1377a), 1:5000 dilution. Cells were either (A) untreated (B) treated with LP
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q05469
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EPR2330
Calculated MW 116598 Da
Gene ID 3991
Other Names Hormone-sensitive lipase, HSL, LIPE
Target/Specificity A phospho-specific peptide corresponding to residues surrounding Serine 855 of human HSL was used as an immunogen. The antibody only detects HSL phosphorylated on Serine 855.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSL Antibody Phospho (pS855) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Cellular Location Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Note=Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation
Research Areas
Citations (0)

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Hormone-sensitive lipase (HSL) is a key enzyme in fatty acid mobilization, overall energy homeostasis, and possibly steroidogenesis. It is acutely controlled through reversible phosphorylation by catecholamines and insulin (1). HSL is the rate-limiting enzyme in hydrolysis of triglycerides in adipose tissue and of cholesteryl esters in steroidogenic tissues including the adrenals, ovaries, testes, and macrophages (2, 3). HSL activity has been positively correlated with free and esterified cholesterol ratios in seminiferous tubules (STf) and interstitial tissue (ITf) enriched fractions, but not with triglyceride levels, during testicular development. HSL is localized to elongated spermatids and Sertoli cells, where its distribution is stage-dependent, and within the cells lining the excurrent ducts of the testis. The HSL protein levels and enzymatic activity in ITf and STf were reported positively correlated with serum testosterone levels during development (4).


1. Holm C, et al. Science 241(4872):1503-6, 1988
2. Li Z, et al. Genomics 24(2):259-65, 1994
3. Osuga J, et al. Proc Natl Acad Sci U S A. 97(2):787-92, 2000
4. Kabbaj O, et al Biology of Reproduction 65:601-612, 2001

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Cat# AJ1377a
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