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JAK3 Antibody

Rabbit Monoclonal IgG

  • WB - JAK3 Antibody AJ1422a
    Western blot analysis on (A) IM-9 and (B) TF-1 cell lysates using anti-JAK3 RabMAb (Cat. #AJ1422a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P52333
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR908(2)
Calculated MW 125099 Da
Gene ID 3718
Other Names Tyrosine-protein kinase JAK3, Janus kinase 3, JAK-3, Leukocyte janus kinase, L-JAK, JAK3
Target/Specificity A synthetic peptide corresponding to residues on the N-terminus in human JAK3 was used as an immunogen.
Dilution WB~~1:10000~20000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsJAK3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name JAK3
Function Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
Cellular Location Endomembrane system; Peripheral membrane protein. Cytoplasm
Tissue Location In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins
Research Areas
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JAK3 is a member of the Janus family (JAK) tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. The JAK family is constitutively associated with cytokine receptors; upon binding to the receptor, the cytokine becomes phosphorylated (1). JAK also phosphorylates the cytokine receptor on tyrosine (Tyr) residues that serve as docking sites for cytoplasmic signal transducers and activators of transcription (STAT) proteins (2). Activated STATs translocate to the nucleus to mediate gene transcription (1). JAK3 is unique among JAK family members because it serves a function in normal lymphocyte development. In addition, JAK3 is the only one that interacts with the common gamma chain of the following Interleukin receptors: IL-2, IL-4, IL-7, IL-9, and IL-15 receptors (3). Loss of JAK3 function causes a severe block in T-cell and natural killer (NK) cell development (4). Defects in JAK3 are a cause of severe combined immunodeficiency (SCID), characterized by an absence of T-cell-mediated cellular immunity (5).


1. Leonard WJ et al. Annu Rev Immunol. 16:293-322, 1998. 2. Vaysberg M et al. Am J Transplant. 9(10):2292-302, 2009. 3. Malabarba M et al. J Biol Chem, 270:9630-9637, 1995. 4. Cornejo MG et al. Blood. 113(12):2746-54, 2009. 5. Pesu M et al. Immunol Rev. 203:127-42, 2005.

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Cat# AJ1422a
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