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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   KID Antibody Phospho (pS427)   

KID Antibody Phospho (pS427)

Rabbit Monoclonal Antibody

  • WB - KID Antibody Phospho (pS427) AJ1428b
    A.Western blot analysis on HeLa cell lysates using anti-Phospho-KID (pS427) RabMAb (Cat. #AJ1428b), 1:2000 dilution. Cells were either (A) untreated (B) treated with aphidicolin + nocodazole
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14807
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR2728Y
Calculated MW 73262 Da
Gene ID 3835
Other Names Kinesin-like protein KIF22, Kinesin-like DNA-binding protein, Kinesin-like protein 4, KIF22, KID, KNSL4
Target/Specificity A phospho-specific peptide corresponding to residues surrounding Serine 427 of human KID was used as an immunogen. This antibody only detects KID phosphorylated on Serine 427.
Dilution WB~~1:1000~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKID Antibody Phospho (pS427) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KIF22
Synonyms KID, KNSL4
Function Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA (By similarity). Plays a role in congression of laterally attached chromosomes in NDC80- depleted cells (PubMed:25743205).
Cellular Location Nucleus. Cytoplasm, cytoskeleton
Tissue Location Expressed in bone, cartilage, joint capsule, ligament, skin, and primary cultured chondrocytes
Research Areas
Citations (0)

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KID (KNSL4; kinesin-like DNA-binding protein) is a member of the kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis (1). KID colocalizes with mitotic chromosomes and is enriched in the kinetochore at anaphase. The N-terminal region of KID shares greater than 35% sequence homology with the motor domains of other kinesins. The C-terminal domain has limited but significant homology to NOD, a Drosophila protein and member of the kinesin family. KID also contains a putative nuclear localization signal (2). It was demonstrated that SIAH, a human homologue of the Drosophila seven in absentia (Sina), is involved in the degradation of KID via the ubiquitin-proteasome pathway (3). It was also reported that KID function is regulated by phosphorylation. Ser427 and Thr463 were identified as M phase-specific phosphorylation sites (4).


1. Song J, et al. Genomics 52(3):374-7, 1998
2. Tokai N, et al. EMBO J. 15(3):457-67. 1996
3. Germani A, et al. Oncogene 19(52):5997-6006, 2000
4. Tokai-Nishizumi N, et al. The EMBO Journal 22: 2091-2103, 2003

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Cat# AJ1428b
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