|Application ||IHC, WB|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||36638 Da|
|Other Names||L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, LDH-H, Renal carcinoma antigen NY-REN-46, LDHB|
|Target/Specificity||A synthetic peptide corresponding to residues in human LDH-B was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LDH-B Antibody (Heart Subunit) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2).
1. Kopperschlager G et al. J Chromatogr B Biomed Appl. 684(1-2):25-49, 1996.
2. Rodriguez S et al. Oncogene 22(12): 1880-1891, 2003
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