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MCM5 Antibody

Rabbit Monoclonal Antibody

  • WB - MCM5 Antibody AJ1464b
    A. Western blot analysis on (A) HeLa (B) MCF7 and (C) Human spleen lysates using anti-MCM5 RabMAb (Cat. #AJ1464b), dilution 1:2000.
  • IHC - MCM5 Antibody AJ1464b
    B. Immunohistochemical analysis of paraffin-embedded human squamous cell cervical carcinoma using anti-MCM5 RabMAb (Cat. #AJ1464b)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P33992
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP2682Y
Calculated MW 82286 Da
Gene ID 4174
Other Names DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46, MCM5, CDC46
Target/Specificity A synthetic peptide corresponding to residues near the N-term of human MCM5 was used as an immunogen.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMCM5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MCM5
Synonyms CDC46
Function Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.
Cellular Location Nucleus. Cytoplasm, cytosol
Citations (0)

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Human nuclear proteins MCM3 and MCM5 have high sequence similarities with the corresponding yeast proteins known to be required for the initiation of genome replication. At 0.5 M NaCl, the structure-bound nuclear protein can be partially solubilized as a dimer composed of MCM3 and the related protein MCM5. MCM3 forms stable complexes with protein MCM5. These MCM3/MCM5 complexes occur as dimers and in high-molecular-mass complexes (approximately 500 kDa). The high-molecular-mass complexes dissociate in 0.5 M NaCl and release MCM3/MCM5 dimers. It has frequently been proposed that the MCM proteins may function as licensing factors for genome replication. Data imply that the active form of an MCM protein is not a monomer, but a protein complex that includes an MCM3/MCM5 dimer (1). Levels of member of the MCM family (MCM2, MCM3, MCM5 and MCM7) gradually increased in a variable manner as KD cells progressed from GO into the G1/S phase. In the GO stage, the amounts of MCM2 and -5 proteins were much lower than those of MCM7 and -3 proteins, suggesting that they are not present in stoichiometric amounts, and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM/P1 complexes (2).


1. Burkhart R, et al. Eur J Biochem 228(2):431-8, 1995
2. Tsuruga H, et al. Biochem Biophys Res 236(1):118-25

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Cat# AJ1464b
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