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MKK5 Antibody

Rabbit Monoclonal Antibody

  • WB - MKK5 Antibody AJ1484a
    A. Western blot analysis on HeLa cell lysate using anti-MKK5 RabMAb (Cat. #AJ1484a).
  • IF - MKK5 Antibody AJ1484a
    B. Immunofluorescent staining of HeLa using anti-MKK5 RabMAb (Cat. #AJ1484a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13163
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP648Y
Calculated MW 50112 Da
Gene ID 5607
Other Names Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, MAP2K5, MEK5, MKK5, PRKMK5
Target/Specificity A synthetic peptide corresponding to residues within human MKK5 was used as an immunogen.
Dilution WB~~1:10000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMKK5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP2K5
Synonyms MEK5, MKK5, PRKMK5
Function Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.
Tissue Location Expressed in many adult tissues. Abundant in heart and skeletal muscle
Research Areas
Citations (0)

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Mitogen-acitvated protein kinase kinase 5 (MKK5) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). The MAPK cascade plays an important role in signaling defense responses. Of the MKK family members, MKK5 exhibits the closest homology to MEK1 and MEK2, with 46% amino acid identity in the catalytic domain (3) MKK has been show to activate extracellular-signal-regulated protein kinase 5 (ERK5). Also, activated ERK5 phosphorylates mitogen- MKK5 extensively at Ser129, Ser137, Ser142 and Ser149, which are located within the region in MKK5 that is thought to interact with ERK5 (3). There have also been findings to that the MEK5 (MKK5)/ERK5 (BMK) pathway may play a role in prostate cancer (5).


1. Han J, et al. J Bio Chem 271:2886-2891, 1996
2. Seger R, et al. FASEB J. 9:726-735, 1995
3. Mody N, et al. FEBS Lett 502:21-24, 2001
4. Mody N, et al. J. Bio Chem 372:567-575, 2001
5. Mehta PB, et al. Oncogene 22:1381-1389, 2003

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Cat# AJ1484a
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