|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||54002 Da|
|Other Names||Macrophage metalloelastase, MME, Macrophage elastase, ME, hME, Matrix metalloproteinase-12, MMP-12, MMP12, HME|
|Target/Specificity||A synthetic peptide corresponding to residues on the C-terminus of human MMP-12 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MMP-12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
|Tissue Location||Found in alveolar macrophages but not in peripheral blood monocytes|
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Provided below are standard protocols that you may find useful for product applications.
Human macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3).
1. Nar H, et al. J Mol Biol 312(4):742-51, 2001.
2. Lang R, et al. J Mol Biol 28(4):731-42, 2001.
3. Curci JA, et al. J Clin Invest 102(11):1900-10, 1998.
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