|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||46529 Da|
|Other Names||Serine/threonine-protein kinase 26, MST3 and SOK1-related kinase, Mammalian STE20-like protein kinase 4, MST-4, STE20-like kinase MST4, Serine/threonine-protein kinase MASK, STK26 (HGNC:18174)|
|Target/Specificity||A synthetic peptide corresponding to residues on the N-terminus of human Mst4 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mst4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mediator of cell growth (PubMed:11641781, PubMed:17360971). Modulates apoptosis (PubMed:11641781, PubMed:17360971). In association with STK24 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation (PubMed:27807006).|
|Cellular Location||Cytoplasm. Golgi apparatus Note=Colocalized with RIPOR1 in the Golgi of serum-starved cells and relocated to cytoplasmic punctae, probably vesicular compartments, along with RIPOR1 upon serum stimulation in a Rho- and PDCD10-dependent manner (PubMed:27807006)|
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Provided below are standard protocols that you may find useful for product applications.
Novel human Ste20-related kinase Mst4 is highly expressed in placenta, thymus, and peripheral blood leukocytes. Mst4 is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation (1). Wild type and C-terminally truncated forms of Mst4 can both induce apoptosis upon overexpression in mammalian cells that is abrogated by CrmA, suggesting involvement of Mst4 in the apoptotic machinery in mammalian cells (2). Mst4 was found to be expressed in prostate carcinoma tumor samples and cell lines. In addition, expression levels appeared to correlate with tumorigenicity and androgen receptor status of the cells. Mst4 kinase activity was stimulated significantly by epidermal growth factor receptor ligands, which are known to promote growth of prostate cancer cells (3).
1. Lin JL, et al. Oncogene 20(45):6559-69, 2001
2. Dan I, et al. J Biol Chem 277(8):5929-39, 2002
3. Sung V, et al. Cancer Res 63(12):3356-63, 2003
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