|Application ||WB, IHC|
|Calculated MW||9072 Da|
|Other Names||NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8, Ubiquitin-like protein Nedd8, NEDD8|
|Target/Specificity||A synthetic peptide corresponding to residues in the N-term of human NEDD8 was used as immunogen. This antibody detects NEDD8 bound to cullins proteins (neddylated cullins) as well as free NEDD8.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NEDD8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.|
|Cellular Location||Nucleus. Note=Mainly nuclear|
|Tissue Location||Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes; E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4).
1. Kumar, S., et al. 1993. Biochem. Biophys. Res. Commun. 195: 393-399
2. Gong, L., et al. (1999) J. Biol. Chem. 274, 12036-12042
3. Kipreos, E.T., et al. 1996. Cell 85: 829-839
4. Katsumi Kito,et al. Biol. Chem., Vol. 276, Issue 23, 20603-20609, June 8, 2001
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