Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Neurofilament-66 Antibody   

Neurofilament-66 Antibody

Rabbit Monoclonal Antibody

  • WB - Neurofilament-66 Antibody AJ1533a
    A. Western blot analysis on SHSY5Y cell lysate using anti-NF-66 RabMAb (Cat. #AJ1533a).
  • IF - Neurofilament-66 Antibody AJ1533a
    B. Immunofluorescent staining of PC 12 cells using anti-NF-66 RabMAb (Cat. #AJ1533a)
  • IHC - Neurofilament-66 Antibody AJ1533a
    C. Positive immunohistochemical staining of paraffin-embedded human brain tissue (A.) using anti-NF-66 RabMAb.
  • IHC - Neurofilament-66 Antibody AJ1533a
    D. Negative immunohistochemical staining of paraffin-embedded (D) human colon (E), Kidney (F) and uterus (D) tissues using NF-66 RabMAb (Cat#. 1674-1). Tissues were stained in parallel on the same Normal Tissue Array
  •  - Neurofilament-66 Antibody AJ1533a
    E. Kidney
  •  - Neurofilament-66 Antibody AJ1533a
    F. Uterus
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16352
Reactivity Human, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP676Y
Calculated MW 55391 Da
Gene ID 9118
Other Names Alpha-internexin, Alpha-Inx, 66 kDa neurofilament protein, NF-66, Neurofilament-66, Neurofilament 5, INA, NEF5
Target/Specificity A synthetic peptide corresponding to residues in the tail domain of human NF-66 was used as immunogen.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeurofilament-66 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name INA
Synonyms NEF5
Function Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Tissue Location Found predominantly in adult CNS.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


Neurofilaments (NF) are cytoplasmic intermediate filaments (IFs) found in neurons. NF-66, also known as α-internexin, is a member of the IF family. Classified as a type IV IF, NF-66 is expressed by most neurons and has been shown to co-assemble with other NF triple proteins (light, medium and heavy NF) (1-2). In the adult brain, NF-66 is expressed at relatively low levels compared to the other NF proteins (3). NF-66 has also been shown to assemble into 10mm diameter IF in vitro.


1. Kaplan MP, Chin SSM, Fliegner KH, Liem RKH: J Neurosci 1990, 10:2735-2748
2. Ching GY, Liem RKH: J Biol Chem 1991, 29:19459-68
3. Ching GY, Liem RKH: J Cell Sci 1998, 111:321-333

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

Cat# AJ1533a
Alternative Products:

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions