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NMDAR2B Antibody Phospho (pS1303)

Rabbit Monoclonal Antibody

  • WB - NMDAR2B Antibody Phospho (pS1303) AJ1547a
    A. Western blot analysis on mouse brain lysates using anti-Phospho-NMDAR2B (pS1303) RabMAb (Cat. #AJ1547a), 1:5000 dilution. Cells were either (A) untreated (B) treated with AP
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13224
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EP1858Y
Calculated MW 166367 Da
Gene ID 2904
Other Names Glutamate receptor ionotropic, NMDA 2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, N-methyl-D-aspartate receptor subunit 3, NR3, hNR3, GRIN2B, NMDAR2B
Target/Specificity A phospho specific peptide corresponding to residues surrounding serine 1303 of human NMDAR2B was used as an immunogen. This antibody detects NMDAR2B phosphorylated at serine 1303.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNMDAR2B Antibody Phospho (pS1303) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms NMDAR2B
Function Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:8768735, PubMed:26919761, PubMed:26875626, PubMed:28126851). Sensitivity to glutamate and channel kinetics depend on the subunit composition (PubMed:8768735, PubMed:26875626). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Contributes to neural pattern formation in the developing brain. Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q00960}. Cell junction, synapse, postsynaptic cell membrane {ECO:0000250|UniProtKB:Q00960}; Multi- pass membrane protein {ECO:0000250|UniProtKB:Q00960}
Tissue Location Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Research Areas
Citations (0)

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The NMDA (N-methyl D-aspartate) receptors in the brain play a critical role in synaptic plasticity, synaptogenesis and excitotoxicity. NMDA R1 and combination of one or more NMDAR2 subunits forms NMDA receptor glutamate-gated ion channels. In this hetromer complex, NMDA R1 binds to co-agonist glycine and NMDA R2 binds to neurotransmitter glutamate (1). Several studies have suggested that protein phosphorylation of NMDA receptors may affect channel function, and additionally regulate these receptors? synaptic trafficking and surface expression. Once activated thru phospholyation, these channels allow movement of Ca2+ and Na+ into the cell and K+ out of the cell. Serine 1303 on NMDAR2B has been identified as a major phospholyation site for CaM kinase II and PKC, regulating kinase-mediated NMNDAR2B/R1 current (2,3). NMDAR has been linked to various neurodegenerative disorders such as schizophrenia, epilepsy, and Alzheimer's disease (4).


1. Tingley WG, et al. Nature 364(6432): 70-3, 1993
2. Strack S, McNeill RB, Colbran RJ. JBC 275(31):23798-806, 2000
3. Liao GY, et al. Mol Pharma 59(5) :960-964, 2001.
4. Chang CG et al, Neurochem Res. 23(11):1371-7, 1998.

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Cat# AJ1547a
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