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Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | O60716 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone Names | EPR2386 |
| Calculated MW | 108170 Da |
| Gene ID | 1500 |
| Other Names | Catenin delta-1, Cadherin-associated Src substrate, CAS, p120 catenin, p120(ctn), p120(cas), CTNND1, KIAA0384 |
| Target/Specificity | A phospho-specific peptide corresponding to residues surrounding Tyrosine 96 of human p120 Catenin was used as an immunogen. The antibody only detects p120 Catenin phosphorylated on Tyrosine 96. |
| Dilution | WB~~1:2500~5000 |
| Format | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | p120 (Catenin) Antibody Phospho (pY96) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CTNND1 |
|---|---|
| Synonyms | KIAA0384 |
| Function | Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). Associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. |
| Cellular Location | Cytoplasm. Nucleus. Cell membrane. Note=Interaction with GLIS2 promotes nuclear translocation (By similarity). Detected at cell-cell contacts (PubMed:15240885, PubMed:17047063). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm (PubMed:17047063). CDH1 enhances cell membrane localization (PubMed:15240885). Isoforms 4A and 1AB are excluded from the nucleus (PubMed:11896187). {ECO:0000250|UniProtKB:P30999, ECO:0000269|PubMed:11896187, ECO:0000269|PubMed:15240885, ECO:0000269|PubMed:17047063} Isoform 2A: Nucleus |
| Tissue Location | Expressed in vascular endothelium. Melanocytes and melanoma cells primarily express the long isoform 1A, whereas keratinocytes express shorter isoforms, especially 3A. The shortest isoform 4A, is detected in normal keratinocytes and melanocytes, and generally lost from cells derived from squamous cell carcinomas or melanomas. The C-terminal alternatively spliced exon B is present in the p120ctn transcripts in the colon, intestine and prostate, but lost in several tumor tissues derived from these organs. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Catenins are proteins that are linked to the cytoplasmic domain of transmembrane cadherins. p120 Catenin is one member of this Armadillo gene family of junctional plaque proteins (1-3). The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be important for cadherins cell-adhesion properties. Recently it became clear that some catenins also mediate nuclear signaling (1). p120 Catenin is implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors.
References
1. Keirsebilck A., et al. Genomics 50(2): 129-146, 1998
2. Aho S., et al. J Cell Sci. 115:1391-1402, 2002
3. van Hengel J., et al. Proc Natl Acad Sci U S A. 96:7980-7985, 1999
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