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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   p27/Kip1 Antibody   

p27/Kip1 Antibody

Rabbit Monoclonal IgG

  • WB - p27/Kip1 Antibody AJ1569b
    Western blot analysis on (A) MCF-7 and (B) SH-SY5Y cell lysates using anti-p27/Kip1 RabMAb (Cat. #AJ1569b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P46414
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPFHCR16a
Calculated MW 22193 Da
Gene ID 12576
Other Names Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b
Target/Specificity A synthetic peptide corresponding to residues in human p27/Kip1 was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsp27/Kip1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Cdkn1b
Function Important regulator of cell cycle progression (PubMed:8033213, PubMed:12972555). Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (By similarity). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes (PubMed:8033213). Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Cellular Location Nucleus. Cytoplasm. Endosome. Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-197, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser- 10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr- 89 (By similarity). Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (PubMed:20228253). {ECO:0000250, ECO:0000269|PubMed:20228253}
Research Areas
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p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. It is an inhibitor of cyclinE-Cdk2 complex, cyclinA-Cdk2 and cyclinD1-Cdk4 (1), and a positive regulator of cyclin D-dependent kinases such as Cdk4 (2). It plays a pivotal role in the control of cell proliferation and the loss of p27/Kip1 function may lead to carcinogenesis (4). The function of p27/Kip1 is regulated by phosphorylation and degradation events. Phosphorylation by hKIS on Serine 10 signals the nuclear export to the cytoplasm (3), while phosphorylation by Cdk2 on Threonine 187 results in ubiquitylation and degradation of p27/Kip1 (2).


1. Polyak K., et al. Cell 78:59-66, 1994. 2. The UniProt Consortium. The Universal Protein Resource (UniProt) Nucleic Acids Res. 36:D190-D195 (2008) 3. Ishida N., et al. The Journal of Biological Chemistry 277(17):14355-14358, 2002 4. Carrano,A.C., et al Nat. Cell Biol., 1, 193-199, 1999.

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Cat# AJ1569b
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