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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   p40 (phox) Antibody   

p40 (phox) Antibody

Rabbit Monoclonal Antibody

  • WB - p40 (phox) Antibody AJ1572a
    A. Western blot analysis on HL-60 cell lysate using anti-p40 RabMAb (Cat. #AJ1572a), dilution 1:50,000.
  • IHC - p40 (phox) Antibody AJ1572a
    B. Immunohistochemical staining of paraffin-embedded spleen tissue using anti-p40 RabMAb (Cat. #AJ1572a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15080
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP2142Y
Calculated MW 39032 Da
Gene ID 4689
Other Names Neutrophil cytosol factor 4, NCF-4, Neutrophil NADPH oxidase factor 4, SH3 and PX domain-containing protein 4, p40-phox, p40phox, NCF4, SH3PXD4
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human p40 was used as an immunogen.
Dilution WB~~1:25000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsp40 (phox) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCF4
Synonyms SH3PXD4
Function Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
Cellular Location Cytoplasm, cytosol. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Membrane; Peripheral membrane protein
Tissue Location Expression is restricted to hematopoietic cells
Research Areas
Citations (0)

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The NADPH oxidase generates superoxide in phagocytic cells. It is important for immunity and its deficiency leads to chronic granulomatous disease (CGD) (1). p40-phox is a newly isolated cytosolic component of the nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase that copurifies with p67-phox. Preliminary evidence indicates that it is a component of the cytosolic complex (2). Maximal activation of NADPH oxidase requires formation of a complex between the p40-phox and p67-phox subunits via association of their PB1 domains. The crystal structure of the p40-phox/p67phox PB1 heterodimer reveals that both domains have a beta grasp topology and that they bind in a front-to-back arrangement through conserved electrostatic interactions between an acidic OPCA motif on p40-phox and basic residues in p67-phox (3).


1. Wientjes FB, et al Biochem J 296(3):557-61, 1993
2. Zhan S, et al. Blood 88(7):2714-21, 1996
3. Wilson MI, et al. Mol Cell 12(1):39-50, 2003

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Cat# AJ1572a
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