|Application ||WB, IF|
|Calculated MW||76785 Da|
|Other Names||Tumor protein 63, p63, Chronic ulcerative stomatitis protein, CUSP, Keratinocyte transcription factor KET, Transformation-related protein 63, TP63, Tumor protein p73-like, p73L, p40, p51, TP63, KET, P63, P73H, P73L, TP73L|
|Target/Specificity||A synthetic peptide corresponding to residues to the C-term of human p63 (p73L) was used as immunogen. This antibody should detect both isoforms 2,9,10,11 and 12.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||p63 (p73L) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||KET, P63, P73H, P73L, TP73L|
|Function||Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.|
|Tissue Location||Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues|
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Provided below are standard protocols that you may find useful for product applications.
p63 (also called KET, p51, p40, AIS, and p73L) (1) is a transcription factor that shares a significant amino acid identity with p53 and p73. p63 and p73 can both activate p53-responsive genes and induce apoptosis upon over- expression (2). p63 has an additional coding region in the C-termini (not found in p53) that can undergo complex alternative splicing giving rise to 3 splice forms (α, β, and γ) (3). Additionally, two isoforms can be found; one containing the N-terminal transactivating domain (TA) or the (ΔN) lacking TA (2). p63 plays a more fundamental role associated with development and differentiation. Mutations in p63, although not as common as p53, may result in the ADULT, AEC and ECC3 syndrome (4-5).
1. Schmale, H., and Bamberger, C. (1997) Oncogene 15, 1363-1367
2. Yang, A., Kaghad, M., Wang, Y., Gillett, E., Fleming, M. D., Dotsch, V., Andrews, N. C., Caput, D., and McKeon, F. (1998) Mol. Cell 2, 305-316
3. Levrero, M., De Laurenzi, V., Costanzo, A., Gong, J., Melino, G., and Wang, J. Y. (1999) Cell Death Differ. 6, 1146-1153
4. Amiel J., Bougeard G., Francannet C., Raclin V., Munnich A., Lyonnet S., Frebourg T.; Eur. J. Hum. Genet. 9:642-645(2001).
5. Akahoshi K., Sakazume S., Kosaki K., Ohashi H., Fukushima Y.; Am. J. Med. Genet. 120:370-373(2003).
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