|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||69623 Da|
|Other Names||Tumor protein p73, p53-like transcription factor, p53-related protein, TP73, P73|
|Target/Specificity||A synthetic peptide corresponding to residues near the transactivation domain of human p73 was used as immunogen. p73 has a predicted molecular weight of 73 kDa but is detected at 63kDa.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||p73 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.|
|Cellular Location||Nucleus. Cytoplasm. Note=Accumulates in the nucleus in response to DNA damage|
|Tissue Location||Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue|
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Provided below are standard protocols that you may find useful for product applications.
p73 is a transcription factor that shares a significant amino acid identity with p53 and p63 (1). p73 and p63 can both activate p53-responsive genes and induce apoptosis upon over- expression (2) Unlike p53, p73 is not induced by DNA damage and is not a target for inactivation by viral oncoproteins (3). Similar to p63, p73 has an additional coding region in the C-terminus (not found in p53) that can undergo complex alternative splicing giving rise to 3 splice forms (α, β, and γ) (4). Activation of p73 requires the presence of functional c-Abl. It has also be found that mutant p53 can bind to and inactivate p73 (5).
1. Kaghad et al. Cell 90: 809-819, 1997.
2. Yang, A., Kaghad, M., Wang, Y., Gillett, E., Fleming, M. D., Dotsch, V., Andrews, N. C., Caput, D., and McKeon, F. (1998) Mol. Cell 2, 305-316
3. Marin MC, Jost CA, DeCaprio JA, Caput D, Kaelin WG Jr. Mol Cell Biol 1998;18:6316-24
4. Levrero, M., De Laurenzi, V., Costanzo, A., Gong, J., Melino, G., and Wang, J. Y. (1999) Cell Death Differ. 6, 1146-1153
5. Marin et al. Nature Genet. 25: 47-54, 2000.
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