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PABP2 Antibody

Rabbit Monoclonal Antibody

  • WB - PABP2 Antibody AJ1580a
    A.Western blot analysis on (A)Raw264.7 , (B)MCF-7 and (C)HeLa cell lysate using anti-PABP2 RabMAb (Cat. #AJ1580a), dilution (A and B) 1:200,000 and (C) 1:1,000,000.
  • IHC - PABP2 Antibody AJ1580a
    B.Immunohistochemical analysis of squamous cell cervical carcinoma using anti-PABP2 RabMAb (Cat. #AJ1580a).
  •  - PABP2 Antibody AJ1580a
    C.Immunofliorescent staining of HeLa cells using anti-PABP2 RabMAb (Cat. #AJ1580a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q86U42
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EP3000Y
Calculated MW 32749 Da
Gene ID 8106
Other Names Polyadenylate-binding protein 2, PABP-2, Poly(A)-binding protein 2, Nuclear poly(A)-binding protein 1, Poly(A)-binding protein II, PABII, Polyadenylate-binding nuclear protein 1, PABPN1, PAB2, PABP2
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human PABP2 was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPABP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms PAB2, PABP2
Function Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (By similarity). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity). Increases the affinity of poly(A) polymerase for RNA (By similarity). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense- mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506). Binds to poly(A) and to poly(G) with high affinity (By similarity). May protect the poly(A) tail from degradation (By similarity). Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484).
Cellular Location Nucleus. Cytoplasm. Nucleus speckle. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus (PubMed:10688363). Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP- sensitive import mechanism (By similarity). Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Colocalizes with SKIP and poly(A) RNA in nuclear speckles (By similarity). Intranuclear filamentous inclusions or "aggregates" are detected in the myocytes of patients; these inclusions contain PABPN1, ubiquitin, subunits of the proteasome and poly(A) RNA. {ECO:0000250|UniProtKB:Q28165, ECO:0000269|PubMed:10688363, ECO:0000269|PubMed:11001936, ECO:0000269|PubMed:11371506, ECO:0000269|PubMed:14663186, ECO:0000269|PubMed:17289661, ECO:0000269|PubMed:27209344}
Tissue Location Ubiquitous.
Citations (0)

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Poly (A)-binding protein II (PABP2) is an abundant nuclear protein that binds with high affinity to nascent poly (A) tails, stimulating their extension and controlling their length. PABP2 is reported to interact with the nuclear export machinery and may therefore contribute to mRNA transport (1). PABP2 stimulates extension of the growing poly (A) tail during the polyadenylation process, and limits the length of the newly synthesized poly (A) tail (2). PABP2 is reported to be detected in filamentous nuclear inclusions, which are the pathological hallmark of oculopharyngeal muscular dystrophy. This suggests that the polyalanine expansions in PABP2 induce a misfolding and aggregation of the protein into insoluble inclusions, similarly to events in neurodegenerative diseases caused by CAG/polyglutamine expansions (3).


1. Calado A, et al. RNA 6(2):245-56, 2000
2. Kim YJ, et al. Hum Mol Genet. 10(11):1129-39, 2001
3. Calado A, et al. Hum Mol Genet. 9(15):2321-8, 2000

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Cat# AJ1580a
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