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PACT Antibody

Rabbit Monoclonal Antibody

  • WB - PACT Antibody AJ1581a
    A.Western blot analysis on (A) K562, (B) Jurkat and (C) HepG2 cell lysates using anti-PRKRA RabMAb (Cat. #AJ1581a), dilution 1:1000.
  • IHC - PACT Antibody AJ1581a
    B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- PRKRA RabMAb (Cat. #AJ1581a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75569
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3224
Calculated MW 34404 Da
Gene ID 8575
Other Names Interferon-inducible double-stranded RNA-dependent protein kinase activator A, PKR-associated protein X, PKR-associating protein X, Protein activator of the interferon-induced protein kinase, Protein kinase, interferon-inducible double-stranded RNA-dependent activator, PRKRA, PACT, RAX
Target/Specificity A synthetic peptide corresponding to residues on the C terminus of human PACT was used as an immunogen.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPACT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms PACT, RAX
Function Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post- transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity).
Cellular Location Cytoplasm, perinuclear region. Cytoplasm.
Citations (0)

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Protein kinase, interferon-inducible double stranded RNA dependent activator (PACT, PRKRA) is a proapoptotic cellular protein activator of PKR, a double-stranded RNA-dependent protein kinase (1). PACT is reported to be stress-activated, and a physiologic activator that couples transmembrane stress signals and protein synthesis (2). Overexpression of PACT in cells causes enhanced sensitivity to stress-induced apoptosis. It is expressed at high levels in colonic epithelial cells, especially as they exit the cell cycle and enter an apoptotic program (3).


1. Patel RC, et al. EMBO J. 17(15):4379-90, 1998
2. Ito T, et al. J Biol Chem. 274(22):15427-32, 1999
3. Gupta V, et al. Am J Physiol Gastrointest Liver Physiol 283(3):G801-G808, 2002

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Cat# AJ1581a
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