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PAK2 Antibody Phospho (pS20)

Rabbit Monoclonal Antibody

  • WB - PAK2 Antibody Phospho (pS20) AJ1584b
    A. Western blot analysis on HeLa cell lysates using anti-Phospho-PAK2 (pS20) RabMAb (Cat. #AJ1584b), 1:20,000 dilution. Cells were either (A) untreated (B) treated with calyculin A
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13177
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR658(2)
Calculated MW 58043 Da
Gene ID 5062
Other Names Serine/threonine-protein kinase PAK 2, Gamma-PAK, PAK65, S6/H4 kinase, p21-activated kinase 2, PAK-2, p58, PAK-2p27, p27, PAK-2p34, p34, C-t-PAK2, PAK2
Target/Specificity A phospho-specific peptide corresponding to residues surrounding Serine 20 of human PAK2 was used as an immunogen.
Dilution WB~~1:10000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAK2 Antibody Phospho (pS20) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAK2
Function Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF- induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase- activated PAK2 phosphorylates MKNK1 and reduces cellular translation.
Cellular Location Serine/threonine-protein kinase PAK 2: Cytoplasm. Note=MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane
Tissue Location Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen
Research Areas
Citations (0)

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p21-activated kinase 2 (PAK2) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). All six PAKs play an important role in diverse cellular processes, including cytoskeletal dynamics, growth/apoptotic signal transduction through MAP kinases, and regulation of transcription factors (4). PAK2 is unique among the PAK isoforms because it is also activated through proteolytic cleavage by caspases or caspase-like proteases (5). Full-length PAK-2 is localized in the cytoplasm, whereas the proteolytic PAK-2p34 fragment translocates to the nucleus. Activation of full-length PAK-2 stimulates cell survival, and proteolytic activation of PAK-2p34 is involved in programmed cell death (6). Phosphorylation within the Nck (Serine 20) and Pak-interacting guanine nucleotide exchange factor (Serine 192 and Serine 197) binding sites blocks the interactions of Pak 2 with certain proteins (7).


1. Manser, E.,et al. Nature 363, 364, 1994
2. Daniels, R. al. Trends Biochem. Sci. 24, 350, 1999
3. Jaffer, Z. et al J. Biochem. Cell Biol. 34, 713-717, 2002
4. Lim, L., et al. Eur. J. Biochem. 242, 171, 1996
5. Walter et al. J. Biol. Chem. 273, 28733-28739, 1998
6. Gnesutta, N., et al. J. Biol. Chem. 276, 14414-14419, 2001
7. Zhan Q, et al. J Immunol. 171(7):3785-93, 2003

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Cat# AJ1584b
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