|Application ||IHC, WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||45518 Da|
|Other Names||cAMP-dependent protein kinase type II-alpha regulatory subunit, PRKAR2A, PKR2, PRKAR2|
|Target/Specificity||A synthetic phospho-peptide corresponding to residues surrounding Ser99 of human PKA RII was used as immunogen. The antibody only detects PKA RII phosphorylated on Serine 99.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PKA, RII Antibody Phospho (pS99) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.|
|Cellular Location||Cytoplasm. Cell membrane. Note=Colocalizes with PJA2 in the cytoplasm and the cell membrane|
|Tissue Location||Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible|
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Provided below are standard protocols that you may find useful for product applications.
PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).
1. Montminy, M. Transcriptional regulation by cyclic AMP. Annu. Rev. Biochem. 66: 807
2. Beebe, S.J. The cAMP-dependent protein kinases and cAMP signal transduction. Semin Cancer Biol. 5: 285
3. Braun, R.K, et al. Phosphorylation of RII subunit and attenuation of cAMP-dependent protein kinase activity by proline-directed protein kinase. Arch Biochem Biophys. 289: 187
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