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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   PKA, RII Antibody Phospho (pS99)   

PKA, RII Antibody Phospho (pS99)

Rabbit Monoclonal Antibody

  • IHC - PKA, RII Antibody Phospho (pS99) AJ1614b
    A. Immunohistochemical analysis of untreated paraffin-embedded lymph node metastatic tissue using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b).
  • IHC - PKA, RII Antibody Phospho (pS99) AJ1614b
    B. Immunohistochemical analysis of Phosphatase-treated paraffin-embedded lymph node metastatic tissue using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b).
  • WB - PKA, RII Antibody Phospho (pS99) AJ1614b
    C. Western blot analysis on K562 cell lysate using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b), 1:2,500 dilution. Cell lysates were either (1) untreated or (2) treated with Alkaline Phosphatase.
  • IF - PKA, RII Antibody Phospho (pS99) AJ1614b
    D. Immunofluorescent analysis of untreated HeLa cells using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b).
  • IF - PKA, RII Antibody Phospho (pS99) AJ1614b
    E. Immunofluorescent analysis of Alkaline Phosphatase-treated HeLa cells using anti-Phospho-PKA RII (pS99) RabMAb (Cat. #AJ1614b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13861
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names E151
Calculated MW 45518 Da
Gene ID 5576
Other Names cAMP-dependent protein kinase type II-alpha regulatory subunit, PRKAR2A, PKR2, PRKAR2
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser99 of human PKA RII was used as immunogen. The antibody only detects PKA RII phosphorylated on Serine 99.
Dilution IHC~~1:250
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPKA, RII Antibody Phospho (pS99) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms PKR2, PRKAR2
Function Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Cellular Location Cytoplasm. Cell membrane. Note=Colocalizes with PJA2 in the cytoplasm and the cell membrane
Tissue Location Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible
Research Areas
Citations (0)

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PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).


1. Montminy, M. Transcriptional regulation by cyclic AMP. Annu. Rev. Biochem. 66: 807
2. Beebe, S.J. The cAMP-dependent protein kinases and cAMP signal transduction. Semin Cancer Biol. 5: 285
3. Braun, R.K, et al. Phosphorylation of RII subunit and attenuation of cAMP-dependent protein kinase activity by proline-directed protein kinase. Arch Biochem Biophys. 289: 187

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Cat# AJ1614b
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