|Application ||WB, IHC|
|Calculated MW||42462 Da|
|Other Names||Protein arginine N-methyltransferase 1, 211-, Histone-arginine N-methyltransferase PRMT1, Interferon receptor 1-bound protein 4, PRMT1, HMT2, HRMT1L2, IR1B4|
|Target/Specificity||A synthetic peptide corresponding to residues in human PRMT1 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PRMT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1 (PubMed:16879614, PubMed:26876602). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15 (PubMed:26575292). Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).|
|Cellular Location||Nucleus. Nucleus, nucleoplasm. Cytoplasm. Cytoplasm, cytosol Note=Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction (By similarity).|
|Tissue Location||Widely expressed (PubMed:11097842). Expressed strongly in colorectal cancer cells (at protein level) (PubMed:28040436). Expressed strongly in colorectal cancer tissues compared to wild-type colon samples (at protein level) (PubMed:28040436). Expressed strongly in colorectal cancer tissues compared to wild-type colon samples (PubMed:28040436)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
PRMT1 is a member of the Protein arginine methyltransferases (PRMTs) family that regulates mRNA processing and maturation by modulating the activity of RNA-binding proteins through methylation (1). PRMTs have been implicated in a variety of processes, including cell proliferation, signal transduction, and protein trafficking. Eleven PRMTs have been identified in humans. Except for PRMT2, 10 and 11, all PRMTs have been shown to catalyze arginine methylation, with PRMT1, 4, 5, 7, 8 and 9 have the ability to also methylate histones (2). PRMT1 appears to be a major type I enzyme in embryonic stem cells, and in wild-type cells, most substrates of the enzyme appear to be maintained in a fully methylated state (3).
1. Scorilas A, et al. Biochem Biophys Res Comm 278(2): 349-59, 2000 2. Pal S, et al. Journal of Cell Physiology 213 (2) 306-315, 2007 3. Pawlak MR, et al. Mol Cell Biol 20(13):4895-69, 2000
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.