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RasGap Antibody

Rabbit Monoclonal Antibody

  • WB - RasGap Antibody AJ1681a
    A. Western blot analysis on Rat brain lysate using anti-RasGAP RabMAb (Cat. #AJ1681a), dilution 1:2,000.
  • IHC - RasGap Antibody AJ1681a
    B. Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-RasGap RabMAb (Cat. #AJ1681a).
  • IF - RasGap Antibody AJ1681a
    C. Immunofluorescent staining of HeLa cells using anti-RasGAP RabMAb (Cat. #AJ1681a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20936
Reactivity Human, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP536Y
Calculated MW 116403 Da
Gene ID 5921
Other Names Ras GTPase-activating protein 1, GAP, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAP, RASA1, GAP, RASA
Target/Specificity A synthetic peptide corresponding to residues near the SH3 domain of human RasGAP was used as immunogen.
Dilution WB~~1:2000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRasGap Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RASA1
Synonyms GAP, RASA
Function Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1.
Cellular Location Cytoplasm.
Tissue Location In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).
Research Areas
Citations (0)

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RasGAP, a regulator of Ras and Rho, stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. RasGAP is a Caspase 3 substrate (1) and acts as both pro- and anti-apoptotic protein depending on the extent of its cleavage by caspases (2). At low levels of caspase activity, RasGAP is cleaved at position 455, generating an N-terminal fragment (fragment N) and a C-terminal fragment (fragment C). Fragment C alone can induce apoptosis, but this response is completely inhibited by fragment N (3). Fragment N appears to be a general blocker of apoptosis downstream of caspase activation because it inhibits caspase 9-induced cell death. At higher caspase activity, fragment N is further processed and Cleavage of fragment N abrogates its protective functions, and hence the second cleavage of RasGAP promotes apoptosis (4).


1. Yang, J.-Y., Michod, D., Walicki, J., and Widmann, C. (2004) Biochem. Pharmacol. 68, 1027
2. Yang, J.-Y., and Widmann, C. (2001) Mol. Cell. Biol. 21, 5346-5358
3. Yang, J.-Y., Michod, D., Walicki, J., Murphy, B. M., Kasibhatla, S., Martin, S., and Widmann, C. (2004) Mol. Cell. Biol. 24, 10425
4. Yang, J. Y., Walicki, J., Michod, D., Dubuis, G., and Widmann, C. (2005) Mol. Biol. Cell, 16, 3511

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Cat# AJ1681a
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