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RSK4 Antibody Phospho (pS235)

Rabbit Monoclonal Antibody

  • WB - RSK4 Antibody Phospho (pS235) AJ1700b
    A. Western blot analysis on Hela cell lysate using anti-RSK4 Phospho (pS235) RabMAb (Cat. #AJ1700b), dilution 1:10,000. Cells were either (A) untreated or (B) treated with Calyculin A.
  • IHC - RSK4 Antibody Phospho (pS235) AJ1700b
    B. Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-RSK4 Phospho (pS235) RabMAb, (Cat. #AJ1700b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UK32
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EP1524Y
Calculated MW 83872 Da
Gene ID 27330
Other Names Ribosomal protein S6 kinase alpha-6, S6K-alpha-6, 90 kDa ribosomal protein S6 kinase 6, p90-RSK 6, p90RSK6, Ribosomal S6 kinase 4, RSK-4, pp90RSK4, RPS6KA6, RSK4
Target/Specificity A synthetic peptide corresponding to residues surrounding serine 235 of human Rsk 4 was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRSK4 Antibody Phospho (pS235) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS6KA6
Synonyms RSK4
Function Constitutively active serine/threonine-protein kinase that exhibits growth-factor-independent kinase activity and that may participate in p53/TP53-dependent cell growth arrest signaling and play an inhibitory role during embryogenesis.
Cellular Location Cytoplasm, cytosol. Nucleus. Note=Predominantly cytosolic
Research Areas
Citations (0)

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RSK4 is a member of the family of 90-kDa ribosomal S6 kinases (1). These proteins are a class of serine/threonine kinases that are activated in response to various extracellular signals, including growth factors, polypeptide hormones and neurotransmitters (2). RSK4 is most abundantly expressed in brain and kidney. The predicted protein of 746 amino acids shows a high level of homology to three previously isolated members of the human RSK family. RSK2 is involved in Coffin-Lowry syndrome and nonspecific MRX. The localization of RSK4 in the interval that is commonly deleted in mentally retarded males together with the high degree of amino acid identity with RSK2 suggests that RSK4 plays a role in normal neuronal development (3).


1. Moller, D.E., et al. Am J Physiol. 266(2 Pt 1): C351
2. Frodin, M., et al. Mol. Cell. Endocrinol. 151: 65
3. Yntema HG, et al. Genomics 62(3):332-43, 1999.

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Cat# AJ1700b
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