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SAM68 Antibody

Rabbit Monoclonal Antibody

  • WB - SAM68 Antibody AJ1704a
    A.Western blot analysis on (A) HeLa and (B) Jurkat cell lysates using anti- SAM68 RabMAb (Cat. #AJ1704a), dilution 1:100,000.
  • IHC - SAM68 Antibody AJ1704a
    B.Immunohistochemivcal analysis of paraffin-embedded human brain tissue using anti- SAM68 RabMAb (Cat. #AJ1704a).
  • IF - SAM68 Antibody AJ1704a
    C.Immunofluorescent staining of HeLa cells using anti-SAM 68 RaMAb (Cat. #AJ1704a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q07666
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3231
Calculated MW 48227 Da
Gene ID 10657
Other Names KH domain-containing, RNA-binding, signal transduction-associated protein 1, GAP-associated tyrosine phosphoprotein p62, Src-associated in mitosis 68 kDa protein, Sam68, p21 Ras GTPase-activating protein-associated p62, p68, KHDRBS1 (HGNC:18116)
Target/Specificity A synthetic peptide corresponding to residues on the N terminus of human SAM68 was used as an immunogen.
Dilution WB~~1:20000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSAM68 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KHDRBS1 (HGNC:18116)
Function Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain- containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species according to (PubMed:22253824). RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). Can regulate CD44 alternative splicing in a Ras pathway-dependent manner (By similarity). In cooperation with HNRNPA1 modulates alternative splicing of BCL2L1 by promoting splicing toward isoform Bcl-X(S), and of SMN1 (PubMed:17371836, PubMed:20186123). Can regulate alternative splicing of NRXN1 and NRXN3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners. In a neuronal activity-dependent manner cooperates synergistically with KHDRBS2/SLIM-1 in regulation of NRXN1 exon skipping at AS4. The cooperation with KHDRBS2/SLIM-1 is antagonistic for regulation of NXRN3 alternative splicing at AS4 (By similarity).
Cellular Location Nucleus. Cytoplasm. Membrane. Note=Predominantly located in the nucleus but also located partially in the cytoplasm
Tissue Location Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.
Research Areas
Citations (0)

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SAM68 (Src-associated protein in mitosis; 68 kDa) is a member of the signal transduction and activation of RNA family of KH domain-containing RNA binding proteins (1). It is a major substrate of the Src tyrosine kinase in mitotic cells, having a tyrosine-rich C-terminal region and six poly-proline (SH3-binding) sites, many of which are located in an amino-terminal region. Once phosphorylated, SAM68 functions as an adaptor protein in signal transduction cascades by binding to SH2- and SH3-domain containing proteins (2). Tyrosine phosphorylation also negatively regulates the nucleic acid binding properties of SAM68 (3).


1. Babic I, et al. Oncogene 23(21):3781-9, 2004
2. Lee J, et al. Gene 240(1):133-47, 1999
3. Barlat I, et al. J Biol Chem. 272(6):3129-32, 1997

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Cat# AJ1704a
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