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Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q06124 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone Names | EP509Y |
| Calculated MW | 68436 Da |
| Gene ID | 5781 |
| Other Names | Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, PTP-2C, SH-PTP2, SHP-2, Shp2, SH-PTP3, PTPN11, PTP2C, SHPTP2 |
| Target/Specificity | A phospho specific peptide corresponding to residues surrounding Tyrosine 582 of human SHP-2. This antibody detects SHP-2 phosphorylated at Tyrosine 582. |
| Dilution | WB~~1:1000~10000 |
| Format | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SHP-2 Antibody Phospho (pY582) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PTPN11 |
|---|---|
| Synonyms | PTP2C, SHPTP2 |
| Function | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73 (PubMed:26742426). |
| Cellular Location | Cytoplasm. Nucleus |
| Tissue Location | Widely expressed, with highest levels in heart, brain, and skeletal muscle. |
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Provided below are standard protocols that you may find useful for product applications.
Background
SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from the cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Overexpression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).
References
1. Ahmad, et al: Proc. Nat. Acad. Sci. 90: 2197-2201, 1993
2. Klingm
3. Xiao et al (1994) J. Biol. Chem. 269, 21244-21248
4. Tartaglia et al.Hum. Genet. 70: 1555-1563, 2002.
5. Tartaglia et al. Nature Genet. 34: 148-150, 2003.
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