Smad5 Antibody
Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB |
---|---|
Primary Accession | Q99717 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Monoclonal |
Clone Names | EP619Y |
Calculated MW | 52258 Da |
Gene ID | 4090 |
Other Names | Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5, SMAD5, MADH5 |
Target/Specificity | A synthetic peptide corresponding to residues near the MH2 domain of human Smad 5 was used as immunogen. |
Dilution | WB~~1:500 |
Format | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Smad5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SMAD5 |
---|---|
Synonyms | MADH5 |
Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). |
Cellular Location | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 |
Tissue Location | Ubiquitous. |

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Provided below are standard protocols that you may find useful for product applications.
Background
Smad 5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided into 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad 5 is directly activated by BMP type Ia or Ib receptors upon BMP-2 stimulation and transduced BMP-2 signals to the nucleus by forming a complex with Smad 4 (2-3).
References
1. Eppert et al. Cell 86: 543-552, 1996
2. Macias-Silva, M., Hoodless, P. A., Tang, S. J., Buchwald, M., and Wrana, J. L. (1998) J. Biol. Chem. 273, 25628-25636
3. Abdollah, S., Mac韆s-Silva, M., Tsukazaki, T., Hayashi, H., Attisano, L., and Wrana, J. L. (1997) J. Biol. Chem. 272, 27678-27685

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